GENEMARK PREDICTIONS Sequence: gi|10955262|ref|NC_002127.1| Escherichia coli O157:H7 str. Sakai plasmid pOSAK1, complete sequence Sequence file: NC_002127.fna Sequence length: 3306 GC Content: 43.41% Window length: 96 Window step: 12 Threshold value: 0.500 --- Matrix: Heuristic, GC = 43 Matrix author: Borodovsky Laboratory - Georgia Tech, School of Biology, Atlanta, GA, USA Matrix order: 2 List of Open reading frames predicted as CDSs, shown with alternate starts (regions from start to stop codon w/ coding function >0.50) Left Right DNA Coding Avg Start end end Strand Frame Prob Prob -------- -------- ---------- ----- ---- ---- 317 736 direct fr 2 0.54 0.10 341 736 direct fr 2 0.54 0.05 389 736 direct fr 2 0.60 0.08 413 736 direct fr 2 0.64 0.03 590 736 direct fr 2 0.68 0.24 605 736 direct fr 2 0.66 0.12 1 141 complement fr 3 0.94 0.80 1 111 complement fr 3 0.97 0.09 971 1351 complement fr 1 0.60 0.97 1348 2388 complement fr 3 0.65 0.83 1348 2229 complement fr 3 0.64 0.09 1348 1989 complement fr 3 0.59 0.20 1348 1884 complement fr 3 0.58 0.34 1348 1866 complement fr 3 0.57 0.72 List of Regions of interest (regions from stop to stop codon w/ a signal in between) LEnd REnd Strand Frame -------- -------- ----------- ----- 2 736 direct fr 2 4 186 complement fr 3 971 1363 complement fr 1 1348 2481 complement fr 3 2874 3125 direct fr 3 -------------------- ABOUT THE MATRIX USED: For details on the model building procedure see: Besemer J. and Borodovsky M. "Heuristic approach to deriving models for gene finding" NAR, 1999, Vol. 27, No. 19, pp. 3911-3920