LOCUS       NC_002252               7258 bp    DNA     circular BCT 09-JAN-2006
DEFINITION  Buchnera aphidicola str. APS (Acyrthosiphon pisum) plasmid pTrp,
            complete sequence.
ACCESSION   NC_002252
VERSION     NC_002252.1  GI:10957099
KEYWORDS    .
SOURCE      Buchnera aphidicola str. APS (Acyrthosiphon pisum)
  ORGANISM  Buchnera aphidicola str. APS (Acyrthosiphon pisum)
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacteriales;
            Enterobacteriaceae; Buchnera.
REFERENCE   1  (sites)
  AUTHORS   Shigenobu,S., Watanabe,H., Hattori,M., Sakaki,Y. and Ishikawa,H.
  TITLE     Genome sequence of the endocellular bacterial symbiont of aphids
            Buchnera sp. APS
  JOURNAL   Nature 407 (6800), 81-86 (2000)
   PUBMED   10993077
REFERENCE   2  (bases 1 to 7258)
  CONSRTM   NCBI Microbial Genomes Annotation Project
  TITLE     Direct Submission
  JOURNAL   Submitted (17-JAN-2000) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AP001070.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..7258
                     /organism="Buchnera aphidicola str. APS (Acyrthosiphon
                     pisum)"
                     /mol_type="genomic DNA"
                     /strain="APS"
                     /isolate="Tokyo1998"
                     /specific_host="Acyrthosiphon pisum"
                     /db_xref="taxon:107806"
                     /plasmid="pTrp"
                     /note="primary endosymbiont of Acyrthosiphon pisum
                     trpEG plasmid"
     gene            1..1566
                     /gene="trpE"
                     /locus_tag="BUpT01"
                     /db_xref="GeneID:1109444"
     CDS             1..1566
                     /gene="trpE"
                     /locus_tag="BUpT01"
                     /EC_number="4.1.3.27"
                     /note="catalyzes the formation of anthranilate and
                     glutamate from chorismate and glutamine"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="NP_057962.1"
                     /db_xref="GI:10957100"
                     /db_xref="GeneID:1109444"
                     /translation="MFLIEKRRKLIQKKANYHSDPTTVFNHLCGSRPATLLLETAEVN
                     KKNNLESIMIVDSAIRVSAVKNSVKITALSENGAEILSILKENPHKKIKFFEKNKSIN
                     LIFPSLDNNLDEDKKIFSLSVFDSFRFIMKSVNNTKRTSKAMFFGGLFSYDLISNFES
                     LPNVKKKQKCPDFCFYLAETLLVVDHQKKTCLIQSSLFGRNVDEKNRIKKRTEEIEKK
                     LEEKLTSIPKNKTTVPVQLTSNISDFQYSSTIKKLQKLIQKGEIFQVVPSRKFFLPCD
                     NSLSAYQELKKSNPSPYMFFMQDEDFILFGASPESSLKYDEKNRQIELYPIAGTRPRG
                     RKKDGTLDLDLDSRIELEMRTNHKELAEHLMLVDLARNDLARICEPGSRYVSDLVKVD
                     KYSHVMHLVSKVVGQLKYGLDALHAYSSCMNMGTLTGAPKVRAMQLIAEYEGEGRGSY
                     GGAIGYFTDLGNLDTCITIRSAYVESGVATIQAGAGVVFNSIPEDEVKESLNKAQAVI
                     NAIKKAHFTMGSS"
     variation       256
                     /gene="trpE"
                     /locus_tag="BUpT01"
                     /note="polymorphism: G or A"
     variation       1215
                     /gene="trpE"
                     /locus_tag="BUpT01"
                     /note="polymorphism: G or C"
     variation       1512
                     /gene="trpE"
                     /locus_tag="BUpT01"
                     /note="polymorphism: G or A"
     gene            1569..2171
                     /gene="trpG"
                     /locus_tag="BUpT02"
                     /db_xref="GeneID:1109446"
     CDS             1569..2171
                     /gene="trpG"
                     /locus_tag="BUpT02"
                     /EC_number="4.1.3.27"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase small subunit"
                     /protein_id="NP_057963.1"
                     /db_xref="GI:10957101"
                     /db_xref="GeneID:1109446"
                     /translation="MANILLLDNIDSFTYNLVEQLRNQKNNVLVYRNTVSIDIIFNSL
                     KKLTHPILMLSPGPSLPKHAGCMLDLIKKVKGDIPIVGICLGHQAIVEAYGGIIGYAG
                     EIFHGKASLIRHDGLEMFEGVPQPLPVARYHSLICNKIPEKFVINSYFEKMIMSVRNN
                     CDRVCGFQFHPESILTTHGDQILEKIIHWASLKYITNKKQ"
     variation       2170
                     /gene="trpG"
                     /locus_tag="BUpT02"
                     /note="polymorphism: A or none"
     gene            3629..5196
                     /gene="trpE2"
                     /locus_tag="BUpT03"
                     /db_xref="GeneID:1109445"
     misc_feature    3629..5196
                     /gene="trpE2"
                     /locus_tag="BUpT03"
                     /note="authentic frameshift; This 1568 nt putative ORF is
                     99 pct identical to trpE"
     gene            5199..5801
                     /gene="trpG2"
                     /locus_tag="BUpT04"
                     /db_xref="GeneID:1109447"
     CDS             5199..5801
                     /gene="trpG2"
                     /locus_tag="BUpT04"
                     /EC_number="4.1.3.27"
                     /note="100 pct identical to trpG
                     BUpT04"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase small subunit"
                     /protein_id="NP_057964.1"
                     /db_xref="GI:10957102"
                     /db_xref="GeneID:1109447"
                     /translation="MANILLLDNIDSFTYNLVEQLRNQKNNVLVYRNTVSIDIIFNSL
                     KKLTHPILMLSPGPSLPKHAGCMLDLIKKVKGDIPIVGICLGHQAIVEAYGGIIGYAG
                     EIFHGKASLIRHDGLEMFEGVPQPLPVARYHSLICNKIPEKFVINSYFEKMIMSVRNN
                     CDRVCGFQFHPESILTTHGDQILEKIIHWASLKYITNKKQ"
     variation       6041
                     /note="polymorphism: T or C"
     variation       6242
                     /note="polymorphism: A or T"
     variation       6993
                     /note="polymorphism: G or A"
     variation       7149
                     /note="polymorphism: A or G"
ORIGIN      
        1 atttttttga ttgaaaagag acgtaaatta atacaaaaaa aggctaatta tcactctgat
       61 cctacaacag tttttaatca tctttgtgga tcaagacccg caacattatt actggaaaca
      121 gcagaagtta ataaaaaaaa taatttagaa agtataatga ttgtcgatag cgcgatacgc
      181 gtctctgctg tcaagaattc agttaagatt actgcgctct ctgaaaacgg agcagagata
      241 ttatctattt taaaagaaaa ccctcataaa aaaataaaat tttttgaaaa aaacaaaagt
      301 atcaacttga tctttccctc tttagacaac aatcttgatg aagataaaaa aattttttct
      361 ttatcagtat ttgattcttt cagattcatt atgaaatctg ttaacaacac aaaaagaaca
      421 agtaaagcaa tgttttttgg cggattgttt tcttatgatc ttatttctaa ttttgaatcg
      481 ttgccgaatg taaagaaaaa acaaaaatgc ccggattttt gtttttattt agcagaaaca
      541 ttacttgttg tagatcatca aaaaaaaaca tgtttgattc aaagcagttt atttggcaga
      601 aatgtagatg aaaaaaatag aattaaaaaa agaacagaag aaatagaaaa gaaactggaa
      661 gaaaaattga catccattcc taaaaataag acaactgtac cagttcaatt gacttccaat
      721 ataagtgatt ttcaatattc atctacaata aaaaaattac aaaaattaat tcaaaaaggt
      781 gaaattttcc aagtcgtgcc ctctcgaaag ttttttttgc cttgtgacaa ttcattatca
      841 gcatatcaag aattaaaaaa aagcaatccc agtccctata tgttttttat gcaagatgaa
      901 gatttcatac tattcggtgc atctccagaa agttctttaa aatatgatga aaaaaataga
      961 caaattgagc tctatcctat tgctggaacc agacccagag gtagaaaaaa agacggaact
     1021 ttagatctcg atttagacag cagaatagaa cttgaaatga gaactaatca taaagaactt
     1081 gctgaacatt tgatgctggt tgacttagca agaaatgatc ttgcacgtat ttgtgagccg
     1141 ggatctagat atgtttccga tctagttaag gtggataaat attcgcacgt gatgcatttg
     1201 gtttctaaag tagtggggca attaaaatat ggtttagatg cgcttcacgc atattcctct
     1261 tgtatgaata tggggacatt aactggtgca cccaaagttc gagcgatgca attaattgct
     1321 gaatatgaag gggaagggag aggaagttac ggcggcgcta taggttattt tactgattta
     1381 ggaaatttag atacttgcat cacaatacgt tcagcttatg tggaatcagg tgtcgcaaca
     1441 attcaagcag gagccggtgt tgtttttaat tcaatacctg aagacgaagt gaaagagagt
     1501 ttaaacaagg cgcaagctgt aataaatgct ataaaaaaag cacattttac aatgggatcc
     1561 tcttaagaat ggcaaatatt ttactgttag acaacataga ctcattcacc tataatttag
     1621 ttgaacaact gagaaatcaa aaaaacaatg ttttagttta tcgtaataca gtgagtattg
     1681 atattatttt caattccctt aaaaaattaa cacatccgat tttaatgctg tcgcccggac
     1741 ctagtttgcc gaaacacgca ggatgcatgt tagatttaat aaaaaaagtc aagggggaca
     1801 ttcctatagt aggaatttgt ttaggtcatc aagcaatagt agaagcgtat ggcggcatta
     1861 tcggatatgc aggtgaaata ttccatggca aagcatcatt aatccgccat gatggtttag
     1921 agatgtttga gggtgtaccg caaccactac ctgttgctcg ataccattcc ttaatttgca
     1981 acaaaattcc ggagaaattt gtcattaatt cctattttga aaaaatgatt atgtctgtaa
     2041 gaaacaattg tgatcgtgtt tgcggctttc aatttcatcc tgaatctatt ttaacaacac
     2101 atggagacca aatattagaa aaaattattc attgggcgtc tttaaaatat atcacaaaca
     2161 agaaacaata aaaaaaaaga ttctactcaa acgagtcgtt tttttaaatt ccatatccct
     2221 ttttataaca ctgaatcctc ctgcgtcatc gcattacaca attcaacatc cagacgtgcc
     2281 tgaaaacaaa aacatacaac aatgattctc atgcaattgg aatgacaaaa tcaaatatac
     2341 acaatgaaaa ttttttaata aaacccgtac cgaaattatt gaaaagaagt tttaaaacag
     2401 tgttaatgtt cccaaaaaca ggaggaaaaa tcacaaaaaa tacattagaa aaaatagaaa
     2461 gtatgaaaaa gagaaaatta ttcaaggaaa ggtgggtgta atttttactt catacaaacc
     2521 acaacaagaa ttgattaaaa ctggtcatcg aggtgaatac agatatcaaa ttaaatgaaa
     2581 gaattggtcg aaatctgtga ctgtcaatac tggaatgtat ctcagaaaat aagtcaaact
     2641 ttatcacgat gggtattttt tcattaatag agattatcaa accgacataa aaaaaggggg
     2701 agcgatttta agtactatgt cacttttgta tgcatgagtc taaatgactc gactttaaat
     2761 cggtcttgtc agcctcataa gcacgctgta tgtgtgtaaa tttgtgctcc attagaaaga
     2821 aaatacattt ggtaaagggg aggagaagac tagcggtgac tgtcggatcc aagagcatca
     2881 tcaatcttct tatagatcgc tcttctatca gttttctaaa aagcagttct caaaatttaa
     2941 gtgaactccc tgtctgaaaa atatatgagt cccggaaaac gcacttcaat caccaataaa
     3001 agataattca tgattctgag acagatgaaa acaaaagttg gacaaacaat gatagagata
     3061 aaatatctcc atataaaaat tttataaaat gaactgttgt cagtcataca aaacaattca
     3121 ataaaaagga aaaaatgaaa atttttttct aaaaagttat ccacagtttc tgtctacaag
     3181 tgaattttaa aaaatatgaa attttaagat aaattccaaa acaaaatact ctaacattat
     3241 gttttttata aaaaaatttt ttttttaatt catacaatac atgttgataa catgttcata
     3301 agtctgggga taagttttgt atatatatac aagacacttt ccgatacata ggaatcttac
     3361 gcggaaaaaa gaaaaacatt aaaaacaata aattaattaa gattagcatt ctttaaaaat
     3421 ccttttaaaa gaaaaaaaca aatacatgta ttatatataa ggtttttaat tgttattgtt
     3481 gttttgtatt atactattaa ttagtatcac tttatttaac atatttatta aatacttaaa
     3541 tatatattat acattttttg taaaatcaaa aatgatatta ttgctttaat ttaaaaagtt
     3601 agttattata aaacgaaaat tagatgaaat ttttttgatt gaaaagagac gtaaattaat
     3661 acaaaaaaag gctaattatc actctgatcc tacaacagtt tttaatcatc tttgtggatc
     3721 aagacccgca acattattac tggaaacagc agaagttaat aaaaaaaata atttagaaag
     3781 tataatgatt gtcgatagcg cgatacgcgt ctctgctgtc aagaattcag ttaagattac
     3841 tgcgctctct gaaaacggag cagagatatt atctatttta aaagaaaacc ctcataaaaa
     3901 aataaaattt tttgaaaaaa acaaaagtat caacttgatc tttccctctt tagacaacaa
     3961 tcttgatgaa gataaaaaaa ttttttcttt atcagtattt gattctttca gattcattat
     4021 gaaatctgtt aacaacacaa aaagaacaag taaagcaatg ttttttggcg gattgttttc
     4081 ttatgatctt atttctaatt ttgaatcgtt gccgaatgta aagaaaaaac aaaaatgccc
     4141 ggatttttgt ttttatttag cagaaacatt acttgttgta gatcatcaaa aaaaaacatg
     4201 tttgattcaa agcagtttat ttggcagaaa tgtagatgaa aaaaatagaa ttaaaaaaag
     4261 aacagaagaa atagaaaaga aactggaaga aaaattgaca tccattccta aaaataagac
     4321 aactgtacca gttcaattga cttccaatat aagtgatttt caatattcat ctacaataaa
     4381 aaaattacaa aaattaattc aaaaaggtga aattttccaa gtcgtgccct ctcgaaagtt
     4441 ttttttgcct tgtgacaatt cattatcagc atatcaagaa ttaaaaaaaa gcaatcccag
     4501 tccctatgtg ttttttatgc aagatgaaga tttcatacta ttcggtgcat ctccagaaag
     4561 ttctttaaaa tatgatgaaa aaaatagaca aattgagctc tatcctattg ctggaaccag
     4621 acccagaggt agaaaaaaag acggaacttt agatctcgat ttagacagca gaatagaact
     4681 tgaaatgaga actaatcata aagaacttgc tgaacatttg atgctggttg acttagcaag
     4741 aaatgatctt gcacgtattt gtgagccggg atctagatat gtttccgatc tagttaaggt
     4801 ggataaatat tcgcacgtga tgcatttggt ttctaaagta gtggggcaat taaaatatgg
     4861 tttagatgcg cttcacgcat attcctcttg tatgaatatg gggacattaa ctggtgcacc
     4921 caaagttcga gcgatgcaat taattgctga atatgaaggg gaagggagag gaagttacgg
     4981 cggcgctata ggttatttta ctgatttagg aaatttagat acttgcatca caatacgttc
     5041 agcttatgtg gaatcaggtg tcgcaacaat tcaagcagga gccggtgttg tttttaattc
     5101 acaatacctg aagacgaagt gaaagagagt ttaaacaagg cgcaagctgt aataaatgct
     5161 ataaaaaaag cacattttac aatgggatcc tcttaagaat ggcaaatatt ttactgttag
     5221 acaacataga ctcattcacc tataatttag ttgaacaact gagaaatcaa aaaaacaatg
     5281 ttttagttta tcgtaataca gtgagtattg atattatttt caattccctt aaaaaattaa
     5341 cacatccgat tttaatgctg tcgcccggac ctagtttgcc gaaacacgca ggatgcatgt
     5401 tagatttaat aaaaaaagtc aagggggaca ttcctatagt aggaatttgt ttaggtcatc
     5461 aagcaatagt agaagcgtat ggcggcatta tcggatatgc aggtgaaata ttccatggca
     5521 aagcatcatt aatccgccat gatggtttag agatgtttga gggtgtaccg caaccactac
     5581 ctgttgctcg ataccattcc ttaatttgca acaaaattcc ggagaaattt gtcattaatt
     5641 cctattttga aaaaatgatt atgtctgtaa gaaacaattg tgatcgtgtt tgcggctttc
     5701 aatttcatcc tgaatctatt ttaacaacac atggagacca aatattagaa aaaattattc
     5761 attgggcgtc tttaaaatat atcacaaaca agaaacaata aaaaaaaaag attctactca
     5821 aacgagtcgt ttttttaaat tccatatccc tttttataac actgaatcct cctgcgtcat
     5881 cgcattacac aattcaacat ccagacgtgc ctgaaaacaa aaacatacaa caatgattct
     5941 catgcaattg gaatgacaaa atcaaatata cacaatgaaa attttttaat aaaaccgtac
     6001 ccgaaattat tgaaaagaag ttttaaaaca gtgttaatgt tcccaaaaac aggaggaaaa
     6061 atcacaaaaa atacattaga aaaaatagaa agtatgaaaa agagaaaatt attcaaggaa
     6121 aggtgggtgt aatttttact tcatacaaac cacaacaaga attgattaaa actggtcatc
     6181 gaggtgaata cagatatcaa attaaatgaa agaattggtc gaaatctgtg actgtcaata
     6241 caggaatgta tctcagaaaa taagtcaaac tttatcacga tgggtatttt ttcattaata
     6301 gagattatca aaccgacata aaaaaagggg ggcgatttta agtactatgt cacttttgta
     6361 tgcatgagtc taaatgactc gactttaaat cggtcttgtc agcctcataa gcacgctgta
     6421 tgtgtgtaaa tttgtgctcc attagaaaga aaatacattt ggtaaagggg aggagaagac
     6481 tagcggtgac tgtcggatcc aagagcatca tcaatcttct tatagatcgc tcttctatca
     6541 gttttctaaa aagcagttct caaaatttaa gtgaactccc tgtctgaaaa atatatgagt
     6601 cccggaaaac gcacttcaat caccaataaa agataattca tgattctgag acagatgaaa
     6661 acaaaagttg gacaaacaat gatagagata aaatatctcc atataaaaat tttataaaat
     6721 gaactgttgt cagtcataca aaacaattca ataaaaagga aaaaaatgaa aatttttttc
     6781 taaaaagtta tccacagttt ctgtctacaa gtgaatttta aaaaatatga aattttaaga
     6841 taaattccaa aacaaaatac tctaacatta tgttttttat aaaaaaattt ttttttaatt
     6901 catacaatac atgttgataa catgttcata agtctgggga taagttttgt atatatatac
     6961 aagacacttt ccgatacata ggaatcttac gcggaaaaaa gaaaaacatt aaaaacaata
     7021 aattaattaa gattagcatt ctttaaaaat ccttttaaaa gaaaaaaaca aatacatgta
     7081 ttatatataa ggtttttaat tgttattgtt gttttgtatt atactattaa ttagtatcac
     7141 tttatttaac atatttatta aatacttaaa tatatattat acattttttg taaaatcaaa
     7201 aatgatatta ttgctttaat ttaaaaagtt agttattata aaacgaaaat tagatgaa
//
