LOCUS NC_005951 20652 bp DNA circular BCT 05-DEC-2007 DEFINITION Staphylococcus aureus subsp. aureus MSSA476 plasmid pSAS, complete sequence. ACCESSION NC_005951 VERSION NC_005951.1 GI:49398098 KEYWORDS . SOURCE Staphylococcus aureus subsp. aureus MSSA476 ORGANISM Staphylococcus aureus subsp. aureus MSSA476 Bacteria; Firmicutes; Bacillales; Staphylococcus. REFERENCE 1 (bases 1 to 20652) AUTHORS Holden,M.T., Feil,E.J., Lindsay,J.A., Peacock,S.J., Day,N.P., Enright,M.C., Foster,T.J., Moore,C.E., Hurst,L., Atkin,R., Barron,A., Bason,N., Bentley,S.D., Chillingworth,C., Chillingworth,T., Churcher,C., Clark,L., Corton,C., Cronin,A., Doggett,J., Dowd,L., Feltwell,T., Hance,Z., Harris,B., Hauser,H., Holroyd,S., Jagels,K., James,K.D., Lennard,N., Line,A., Mayes,R., Moule,S., Mungall,K., Ormond,D., Quail,M.A., Rabbinowitsch,E., Rutherford,K., Sanders,M., Sharp,S., Simmonds,M., Stevens,K., Whitehead,S., Barrell,B.G., Spratt,B.G. and Parkhill,J. TITLE Complete genomes of two clinical Staphylococcus aureus strains: evidence for the rapid evolution of virulence and drug resistance JOURNAL Proc. Natl. Acad. Sci. U.S.A. 101 (26), 9786-9791 (2004) PUBMED 15213324 REFERENCE 2 (bases 1 to 20652) CONSRTM NCBI Genome Project TITLE Direct Submission JOURNAL Submitted (29-JUN-2004) National Center for Biotechnology Information, NIH, Bethesda, MD 20894, USA REFERENCE 3 (bases 1 to 20652) AUTHORS Holden,M.T.G. TITLE Direct Submission JOURNAL Submitted (23-JUN-2004) Submitted on behalf of the Pathogen Sequencing Unit, Sanger Institute, Wellcome Trust Genome Campus, Hinxton, Cambridge CB10 1SA, E-mail: mh3@sanger.ac.uk COMMENT PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571858. COMPLETENESS: full length. FEATURES Location/Qualifiers source 1..20652 /organism="Staphylococcus aureus subsp. aureus MSSA476" /mol_type="genomic DNA" /strain="MSSA476" /sub_species="aureus" /db_xref="taxon:282459" /plasmid="pSAS" gene 12..638 /locus_tag="pSAS01" /db_xref="GeneID:2853411" CDS 12..638 /locus_tag="pSAS01" /note="Similar to Lactococcus lactis hypothetical ATP binding protein TR:Q9L650 (EMBL:AF242367) (207 aa) fasta scores: E(): 9.5e-26, 49.75% id in 207 aa, and to Streptococcus pneumoniae hypothetical protein TR:Q9ZHB1 (EMBL:AF068902) (213 aa) fasta scores: E(): 3.2e-24, 45.23% id in 210 aa" /codon_start=1 /transl_table=11 /product="putative ABC transporter ATP binding protein" /protein_id="YP_031698.1" /db_xref="GOA:Q6G5W1" /db_xref="InterPro:IPR003439" /db_xref="InterPro:IPR003593" /db_xref="UniProtKB/TrEMBL:Q6G5W1" /db_xref="GI:49398099" /db_xref="GeneID:2853411" /translation="MIKLENVFVKKGNKNILDGCNFNFEKGKSYALVGESGAGKSTLL NIIAGFEDVSQGSIYIEDKLLKKKVDFYRYTLGYLFQNFALLENQTISQNLDLALKFK KNKKDNMNLKKEVLKKVGLDLDIKRIVSSLSGGEQQRVALARLILKDPKIILADEPTG SLDTKNGKIVIDLLLKLLDENKTMIVVTHDLELAKRFDVIVNISELRS" misc_feature 90..629 /locus_tag="pSAS01" /note="Pfam match to entry PF00005 ABC_tran, ABC transporter, score 191.1, E-value 1.1e-54" misc_feature 111..134 /locus_tag="pSAS01" /note="PS00017 ATP/GTP-binding site motif A (P-loop)." misc_feature 405..449 /locus_tag="pSAS01" /note="PS00211 ABC transporters family signature." gene 876..2144 /locus_tag="pSAS02" /pseudo /db_xref="GeneID:2853417" misc_feature join(876..1178,1182..1502,1506..2144) /locus_tag="pSAS02" /note="Similar to Staphylococcus aureus plasmid pT181 recombination enzyme SW:PRE3_STAAU (P03864) (413 aa) fasta scores: E(): 1.3e-64, 55.92% id in 422 aa, and to Staphylococcus cohnii recombination/mobilization protein Pre TR:O87277 (EMBL:AF015628) (420 aa) fasta scores: E(): 9.9e-28, 31.64% id in 433 aa. Contains nonsense mutations (amber) after codons 101 and 209;putative plasmid recombination enzyme (pseudogene)" /pseudo /db_xref="PSEUDO:CAG43228.1" misc_feature 876..1466 /locus_tag="pSAS02" /note="Pfam match to entry PF01076 Mob_Pre, Plasmid recombination enzyme, score 326.3, E-value 2.1e-95" /pseudo gene complement(2179..2487) /locus_tag="pSAS03" /db_xref="GeneID:2853414" CDS complement(2179..2487) /locus_tag="pSAS03" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_031699.1" /db_xref="UniProtKB/TrEMBL:Q6G5W0" /db_xref="GI:49398100" /db_xref="GeneID:2853414" /translation="MNIHSHLKTFIHNKLLYYQCQTIKGVQLFKTLLLCGKNTPFMWQ KHSFYVAKTLLLCGKNTPLGIEIPCCSRAEQAPKKVFKKIYKNILKSSRGLPIFHPKK " gene 2527..3387 /locus_tag="pSAS04" /db_xref="GeneID:2853422" CDS 2527..3387 /locus_tag="pSAS04" /note="Similar to Neisseria gonorrhoeae replication protein SW:REPA_NEIGO (P17492) (328 aa) fasta scores: E(): 6.7e-08, 22.09% id in 258 aa. Identical to Staphylococcus aureus plasmid pN315 replication protein Rep TR:Q9AC64 (EMBL:AP003139) (286 aa) fasta scores: E(): 6.4e-100, 100% id in 286 aa" /codon_start=1 /transl_table=11 /product="replication protein" /protein_id="YP_031700.1" /db_xref="GOA:Q6G5V9" /db_xref="InterPro:IPR000525" /db_xref="InterPro:IPR011991" /db_xref="UniProtKB/TrEMBL:Q6G5V9" /db_xref="GI:49398101" /db_xref="GeneID:2853422" /translation="MTGETVVYKNEMNLVPLRRFTATEINLFFAMCNKLKEQDTNTLR LSFDELKKLSNYSPETRNINRFANDLDNVYKKMLNLTIRYEDDDVIERFVLFNHYRIH KREQYLEISTSSNLKHILNSITNNFTKFELKEMTRLKSTYSKNMFRLLKQYKHTGYLK IHIDDFKNRLDIPKSYRMTDINKNVFKPIIIELGSIFNNLTINKIKAKKGRKIEWIEF TFDAEKRIHNKRQPQMSKIDKSRQYVRREKTPKWLEERSYEKQPQKDYDPQLEKERED FLKQLELNWE" gene 3474..3815 /locus_tag="pSAS05" /db_xref="GeneID:2853420" CDS 3474..3815 /locus_tag="pSAS05" /note="Similar to Lactococcus lactis transcriptional regulator LL0007 TR:Q9CJI6 (EMBL:AE006240) (107 aa) fasta scores: E(): 5e-09, 36.6% id in 112 aa. N-terminal region is highly similar to Staphylococcus aureus plasmid pN315 hypothetical protein SAP028 TR:Q9AC63 (EMBL:AP003139) (79 aa) fasta scores: E(): 1.4e-25, 98.73% id in 79 aa" /codon_start=1 /transl_table=11 /product="putative regulatory protein" /protein_id="YP_031701.1" /db_xref="GOA:Q6G5V8" /db_xref="InterPro:IPR001387" /db_xref="UniProtKB/TrEMBL:Q6G5V8" /db_xref="GI:49398102" /db_xref="GeneID:2853420" /translation="MKLAEAIKEQRELKGWSQEELANILKVSRQSVSKWESAKNYPSL DILIAMSDLFGISLEHLIKGDARFKKVILEGNYRESNRVPSIVDFLYDFWWLFFPVAG FLVWAIHSFMG" misc_feature 3492..3656 /locus_tag="pSAS05" /note="Pfam match to entry PF01381 HTH_3, Helix-turn-helix, score 70.5, E-value 2.1e-18" gene 3834..4073 /locus_tag="pSAS06" /db_xref="GeneID:2853413" CDS 3834..4073 /locus_tag="pSAS06" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_031702.1" /db_xref="UniProtKB/TrEMBL:Q6G5V7" /db_xref="GI:49398103" /db_xref="GeneID:2853413" /translation="MHIRQYEKGKYFYDTLKCIDKNRRVYKRQYEIYTKSNKNFVCKR TKPRTIRRSSGFFGELALIVYIIFYHVYGANNQIT" misc_feature 3990..4049 /locus_tag="pSAS06" /note="1 probable transmembrane helix predicted for pSAS.06 by TMHMM2.0 at aa 53-72" gene 4274..4459 /locus_tag="pSAS07" /db_xref="GeneID:2853416" CDS 4274..4459 /locus_tag="pSAS07" /note="Poor database matches. Similar to Staphylococcus aureus plasmid pN315 hypothetical protein SAP029 TR:Q9AC61 (EMBL:AP003139) (61 aa) fasta scores: E(): 1.1e-25, 98.36% id in 61 aa" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_031703.1" /db_xref="UniProtKB/TrEMBL:Q6G5V6" /db_xref="GI:49398104" /db_xref="GeneID:2853416" /translation="MFGDQRQEATKYVIKEGYQDIYFLNKNGEWYYFEVRSVWRGKHI IRVKDGLLGWRKEIVTE" gene complement(4678..5421) /locus_tag="pSAS08" /db_xref="GeneID:2853412" CDS complement(4678..5421) /locus_tag="pSAS08" /note="Similar to Staphylococcus aureus plasmid pSK1 replication-associated protein Orf245 TR:Q9L8I7 (EMBL:AF203376) (245 aa) fasta scores: E(): 4e-18, 45.59% id in 261 aa, and to Staphylococcus haemolyticus plasmid pNVH97A hypothetical protein TR:Q9K4M8 (EMBL:AJ400722) (245 aa) fasta scores: E(): 1.5e-40, 63.56% id in 247 aa" /codon_start=1 /transl_table=11 /product="replication-associated protein" /protein_id="YP_031704.1" /db_xref="GOA:Q6G5V5" /db_xref="InterPro:IPR007489" /db_xref="UniProtKB/TrEMBL:Q6G5V5" /db_xref="GI:49398105" /db_xref="GeneID:2853412" /translation="MKSVKRLSEELGVSKQTIFNNIKRLNIETIKQENTSFIKEDTDI EKIIQRVNENKKKYGFESATEDKQKKESDNINFESKSDAQIVEILKNQINTLNNQIEK QESRHETTIEFYRKELQERSKLLENQQVLALESNKKIQKLENQLEKEKHLNYPFDTST NARQNVDAQEKTYTTSPVNINRDQEETKETEIQYKDISGSQSDESTQGEEAQREDVSA NPNDNDSDIEEKSEETEAKKGFWSRLFGN" gene 5832..6333 /locus_tag="pSAS09" /pseudo /db_xref="GeneID:2853415" misc_feature 5832..6333 /locus_tag="pSAS09" /note="Similar to the N-terminal regions of Staphylococcus aureus plasmid pSV41 putative replication initiation protein Rep TR:O87367 (EMBL:AF051917) (319 aa) fasta scores: E(): 5.6e-27, 56.29% id in 151 aa, and Enterococcus faecalis plasmid pCF10 regulatory protein PrgW TR:Q51642 (EMBL:L14285) (333 aa) fasta scores: E(): 1.7e-18, 44.64% id in 168 aa;putative replication initiation protein (fragement)" /pseudo /db_xref="PSEUDO:CAG43243.1" gene 6330..6793 /locus_tag="pSAS10" /pseudo /db_xref="GeneID:2853408" misc_feature join(6330..6655,6659..6793) /locus_tag="pSAS10" /note="Similar to the C-terminal regions Staphylococcus aureus plasmid pC55s putative replication protein Rep TR:Q938S2 (EMBL:AY048756) (331 aa) fasta scores: E(): 1.8e-20, 63.63% id in 132 aa, and to Staphylococcus epidermidis Orf334 orf334 TR:Q53999 (EMBL:U15783) (334 aa) fasta scores: E(): 2e-20, 62.04% id in 137 aa;putative replication protein (fragment)" /pseudo /db_xref="PSEUDO:CAG43244.1" misc_feature 6396..6791 /locus_tag="pSAS10" /note="Pfam match to entry PF01446 Rep, Replication protein, score -29.1, E-value 1e-08" /pseudo gene 7998..8627 /gene="cadD" /locus_tag="pSAS11" /db_xref="GeneID:2853406" CDS 7998..8627 /gene="cadD" /locus_tag="pSAS11" /note="Almost identical to Staphylococcus aureus plasmid pRW001 putative cadmium resistance protein CadD TR:O05469 (EMBL:AF134905) (209 aa) fasta scores: E(): 2.9e-70, 99.04% id in 209 aa. Similar to Neisseria meningitidis cadmium resistance protein NMB1955 TR:Q9JXN6 (EMBL:AE002544) (208 aa) fasta scores: E(): 3.9e-41, 56.93% id in 209 aa" /codon_start=1 /transl_table=11 /product="cadmium resistance transporter" /protein_id="YP_031705.1" /db_xref="InterPro:IPR004676" /db_xref="UniProtKB/TrEMBL:Q6G5V4" /db_xref="GI:49398106" /db_xref="GeneID:2853406" /translation="MRCIMIQTVVAAAVLYIATAVDLLVILLIFFARAKTRKEYRNIY VGQYLGSIILILVSLFLAFVLNYVPEKWILGLLGLIPIYLGIKVAIYDDCEGEKRAKK ELNEKGLSKLVGTVAIVTIASCGADNIGLFVPYFVTLSVTNLLLTLFVFLILIFFLVF TAQKLANIPGIGEIVEKFSRWIMAIIYIALGLFIIIENDTIQTILGFIF" misc_feature order(8025..8093,8130..8198,8211..8270,8331..8399, 8409..8477,8538..8606) /gene="cadD" /locus_tag="pSAS11" /note="6 probable transmembrane helices predicted for pSAS.11 by TMHMM2.0 at aa 10-32, 45-67, 72-91, 112-134, 138-160 and 181-203" misc_feature 8043..8618 /gene="cadD" /locus_tag="pSAS11" /note="Pfam match to entry PF03596 Cad, Cadmium resistance transporter, score 434.5, E-value 5.8e-128" gene 8646..8993 /gene="cadC" /locus_tag="pSAS12" /db_xref="GeneID:2853410" CDS 8646..8993 /gene="cadC" /locus_tag="pSAS12" /note="Similar to Staphylococcus aureus plasmid pI258 cadmium efflux system accessory protein CadC SW:CADC_STAAU (P20047) (122 aa) fasta scores: E(): 7.8e-13, 42.45% id in 106 aa, and to Staphylococcus aureus plasmids pN315 and pC55s, putative regulator of cadB, CadX TR:Q9AC84 (EMBL:AP003139) (115 aa) fasta scores: E(): 1.1e-41, 99.13% id in 115 aa" /codon_start=1 /transl_table=11 /product="putative cadmium efflux system regulatory protein" /protein_id="YP_031706.1" /db_xref="GOA:Q6G5V3" /db_xref="InterPro:IPR001845" /db_xref="InterPro:IPR011991" /db_xref="UniProtKB/TrEMBL:Q6G5V3" /db_xref="GI:49398107" /db_xref="GeneID:2853410" /translation="MSYENTCDVICVHEDKVNNALSFLEDDKSKKLLNILEKICDEKK LKIILSLIKEDELCVCDISLILKMSVASTSHHLRLLYKNEVLDFYKDGKMAYYFIKDD EIREFFSKNQEGF" misc_feature 8751..8987 /gene="cadC" /locus_tag="pSAS12" /note="Pfam match to entry PF01022 HTH_5, Bacterial regulatory protein, arsR family, score 95.0, E-value 9e-26" misc_feature 8817..8873 /gene="cadC" /locus_tag="pSAS12" /note="PS00846 Bacterial regulatory proteins, arsR family signature." gene 9466..10440 /locus_tag="pSAS13" /db_xref="GeneID:2853423" CDS 9466..10440 /locus_tag="pSAS13" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_031707.1" /db_xref="InterPro:IPR002129" /db_xref="UniProtKB/TrEMBL:Q6G5V2" /db_xref="GI:49398108" /db_xref="GeneID:2853423" /translation="MSNTKIKYQIFISSTYLDLIEERQAVVEAVLNSNNIPAGMELFN SSSKSQWEIITQWINESDAVILLLGSSYGTIDSEENISYTEMEYNFAKSLGKPIYVFE MNKASTESAEKSKKLDEFKTKVKENRLISPNINDISELKSKVSQTLIQSVPSEIKENS GWIRVQDCGKNVKKIKKLDAIQRDLDTYDFLKEELYKIFMYVKTFYFQHTPFDDSYIE DIENYLLNYENNPHKSFMKIKVYDYKNFLKHLKNILILIKQGKKPLAQAGHFKLSFYE EDLKLLQKNYKATLVDIKGLNKHFYKECDRCLKAYNIFIEGINKHLYE" misc_feature 10282..10347 /locus_tag="pSAS13" /note="PS00392 DDC / GAD / HDC / TyrDC pyridoxal-phosphate attachment site." gene complement(10560..11375) /locus_tag="pSAS14" /db_xref="GeneID:2853424" CDS complement(10560..11375) /locus_tag="pSAS14" /note="Similar to Staphylococcus aureus Tn552 transposable element potential ATP-binding protein SW:ATBP_STAAU (P18179) (271 aa) fasta scores: E(): 7.4e-99, 95.94% id in 271 aa, and to Listeria innocua plasmid pLI100 hypothetical protein Pli0057 TR:Q926K3 (EMBL:AL592102) (285 aa) fasta scores: E(): 2.8e-33, 51.97% id in 279 aa" /codon_start=1 /transl_table=11 /product="potential ATP-binding protein" /protein_id="YP_031708.1" /db_xref="GOA:Q6G5V1" /db_xref="UniProtKB/TrEMBL:Q6G5V1" /db_xref="GI:49398109" /db_xref="GeneID:2853424" /translation="MTKNQNFIETKEYKRFAEFCDACIKYQYIGICYGQPGVGKTLSS RYYTNWDTIEKQVNHRGWEDLASKTTDDILSVDKIFYNAPAEKQTKLRNDLYSITAII DLGQKLHVVNKYGHEHSKHYSDMFKYIDLIIVDEIDRLKVQHLEQLRAIYDEHNLAMI FIGMPGIEKKLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKCQDLGFDLKLEDFTDY EAITTIIKITKGNFRLIHRLFAQIDRIMDINGLDKISTEVVETARDSLVIGIR" misc_feature complement(11253..11276) /locus_tag="pSAS14" /note="PS00017 ATP/GTP-binding site motif A (P-loop)." gene complement(11368..12811) /locus_tag="pSAS15" /pseudo /db_xref="GeneID:2853404" misc_feature complement(11368..12811) /locus_tag="pSAS15" /note="Similar to Staphylococcus aureus transposon Tn552 transposase SW:TRA3_STAAU (P18416) (480 aa) fasta scores: E(): 3.1e-176, 91.06% id in 481 aa, and to Listeria innocua plasmid pLI100 hypothetical protein Pli0058 TR:Q926K2 (EMBL:AL592102) (478 aa) fasta scores: E(): 3.5e-93, 49.58% id in 484 aa. Contains a frameshift after codon 207. Frameshift occurs at a poly C hexamer;putative transposase (pseudogene)" /pseudo /db_xref="PSEUDO:CAG43255.1" misc_feature complement(11795..12349) /locus_tag="pSAS15" /note="Pfam match to entry PF00665 rve, Integrase core domain, score 106.6, E-value 3e-29" /pseudo gene complement(12783..13376) /gene="tnpR" /locus_tag="pSAS16" /note="synonym: binL" /db_xref="GeneID:2853421" CDS complement(12783..13376) /gene="tnpR" /locus_tag="pSAS16" /note="Similar to Staphylococcus aureus transposon Tn552 resolvase TnpR SW:BINL_STAAU (P18358) (197 aa) fasta scores: E(): 9.2e-71, 95.93% id in 197 aa, and to Staphylococcus epidermidis resolvase/integrase BinR TR:Q93SQ9 (EMBL:AY028779) (192 aa) fasta scores: E(): 6.9e-66, 90.62% id in 192 aa" /codon_start=1 /transl_table=11 /product="transposon resolvase" /protein_id="YP_031709.1" /db_xref="GOA:Q6G5V0" /db_xref="InterPro:IPR006118" /db_xref="InterPro:IPR006119" /db_xref="InterPro:IPR006120" /db_xref="UniProtKB/TrEMBL:Q6G5V0" /db_xref="GI:49398110" /db_xref="GeneID:2853421" /translation="MKIGYARVSTGLQNLNLQEDRLNTYGCEKIFNDHMSGSKSKRPG LDKAIEFARSGDTIVVWRLDRLGRNMEDLITLVNELNERGVSFHSLEENITMDKSSST GQLLFHLFAAFAEFERNLILERSSAGRIAARARGRYGGRPEKLNQKDLNLLKTLYDNG TPIKTIAEQWHVSRTTIYRYLNKLEDKEDEKQGEVSN" misc_feature complement(12825..12959) /gene="tnpR" /locus_tag="pSAS16" /note="Pfam match to entry PF02796 HTH_7, Helix-turn-helix domain of resolvase, score 72.4, E-value 5.9e-19" misc_feature complement(12963..13373) /gene="tnpR" /locus_tag="pSAS16" /note="Pfam match to entry PF00239 resolvase, Resolvase, N terminal domain, score 284.6, E-value 7.8e-83" misc_feature complement(13176..13214) /gene="tnpR" /locus_tag="pSAS16" /note="PS00398 Site-specific recombinases signature 2." misc_feature complement(13338..13364) /gene="tnpR" /locus_tag="pSAS16" /note="PS00397 Site-specific recombinases active site." gene complement(13640..14020) /gene="blaI" /locus_tag="pSAS17" /note="synonym: penI" /db_xref="GeneID:2853407" CDS complement(13640..14020) /gene="blaI" /locus_tag="pSAS17" /note="Highly similar to Staphylococcus aureus plasmid pI258 penicillinase repressor BlaI SW:BLAI_STAAU (P18415) (126 aa) fasta scores: E(): 3.4e-39, 99.2% id in 126 aa. Identical to Staphylococcus haemolyticus plasmids pNVH97A and pNVH96 beta-lactamase repressor BlaI TR:Q9K4N1 (EMBL:AJ400722) (126 aa) fasta scores: E(): 1e-39, 100% id in 126 aa" /codon_start=1 /transl_table=11 /product="penicillinase repressor" /protein_id="YP_031710.1" /db_xref="GOA:Q6G5U9" /db_xref="InterPro:IPR005650" /db_xref="InterPro:IPR011585" /db_xref="UniProtKB/TrEMBL:Q6G5U9" /db_xref="GI:49398111" /db_xref="GeneID:2853407" /translation="MANKQVEISMAEWDVMNIIWDKKSVSANEIVVEIQKYKEVSDKT IRTLITRLYKKEIIKRYKSENIYFYSSNIKEDDIKMKTAKTFLNKLYGGDMKSLVLNF AKNEELNNKEIEELRDILNDISKK" gene complement(14010..15767) /gene="blaR1" /locus_tag="pSAS18" /db_xref="GeneID:2853425" CDS complement(14010..15767) /gene="blaR1" /locus_tag="pSAS18" /note="Similar to Staphylococcus aureus Tn552 transposable element regulatory protein BlaR1 SW:BLAR_STAAU (P18357) (585 aa) fasta scores: E(): 7.1e-205, 94.01% id in 585 aa, and to Staphylococcus haemolyticus plasmid pNVH96 beta-lactamase inducer protein BlaR1 TR:Q9F2I0 (EMBL:AJ302698) (585 aa) fasta scores: E(): 7.9e-203, 92.99% id in 585 aa" /codon_start=1 /transl_table=11 /product="putative beta-lactamase regulatory protein" /protein_id="YP_031711.1" /db_xref="GOA:Q6G5U8" /db_xref="InterPro:IPR000585" /db_xref="InterPro:IPR001460" /db_xref="InterPro:IPR008756" /db_xref="UniProtKB/TrEMBL:Q6G5U8" /db_xref="GI:49398112" /db_xref="GeneID:2853425" /translation="MAKLLIMSVVSFCFIFLLLVFFRYILKRYFNYSLNYKVWYLTVL AGLIPFIPIKFSFIKFNNVNNQAPTVESKSHDLNHNIITTKPVQEFATDIHKFNWDSI DNICTVIWIVLVIILSFKFLKALLYLKYLKKQSLYLNENEKNKIDTILFNHQYKKNIV IRKAEAIQSPITFWYGKYIILIPSSYFKSVIDKRLKYIILHEYAHAKNRDTLHLIIFN IFSIIMSYNPLVHIVKRKIIHDNEVEADRFVLNNINKNEFKTYAESIMDSVLKTSFFN KNILSHSFNGKKSLLKNRLINIKEADLKKQSKLILIFICIFTFFIMIIQSQFLMGQSL TDYNYKKPLQSDYQILDESKNFGSNSGSFVMYSMKKDKYYIYNEKESRKRYSPDSTYK IYLALFGLDRHIISDKNSRMSWNHKHYLFESWNKEQDLNTAMQNSVNWYFERISNQIP KNYTAAQLKQLNYGNENLGSYKSYWMEDSLKISNLEQVIVFKNMMEQNNHFSKKAKNQ LSSSLLIKKNEKYELYGKTGTGIVNGKYNNGWFVGYVITNHDKYYFATHLSDGNPSGK NAELISEKILKGMGVLNGQ" misc_feature complement(14019..14777) /gene="blaR1" /locus_tag="pSAS18" /note="Pfam match to entry PF00144 beta-lactamase, Beta-lactamase, score 132.5, E-value 4.8e-37" misc_feature complement(join(14775..14840,15069..15134,15207..15272, 15387..15452,15588..15653,15690..15755)) /gene="blaR1" /locus_tag="pSAS18" /note="6 probable transmembrane helices predicted for pSAS.18c by TMHMM2.0 at aa 4-26, 38-60, 105-127, 165-187, 211-233 and 309-331" gene 15874..16719 /gene="blaZ" /locus_tag="pSAS19" /db_xref="GeneID:2853409" CDS 15874..16719 /gene="blaZ" /locus_tag="pSAS19" /EC_number="3.5.2.6" /note="Similar to Staphylococcus aureus beta-lactamase precursor BlaZ SW:BLAC_STAAU (P00807) (281 aa) fasta scores: E(): 8.6e-94, 97.5% id in 281 aa, and to Enterococcus faecalis beta-lactamase BlaZ TR:AAA24777 (EMBL:M60253) (281 aa) fasta scores: E(): 8.6e-94, 97.5% id in 281 aa" /codon_start=1 /transl_table=11 /product="beta-lactamase precursor" /protein_id="YP_031712.1" /db_xref="GOA:Q6G5U7" /db_xref="InterPro:IPR000871" /db_xref="InterPro:IPR001466" /db_xref="UniProtKB/TrEMBL:Q6G5U7" /db_xref="GI:49398113" /db_xref="GeneID:2853409" /translation="MKKLIFLIVMALVLSACNSNSSHAKELNDLEKKYNAHIGVYALD TKSGKEVKFNSDKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK YVGKDITLKELIEASMTYSDNTANNKIIKEIGGIKKVKQRLKELGDKVTNPVRYEIEL NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMFNNKNGDTLIKDGVPK DYKVADKSGQAITYASRNDVAFVYPKGQSEPIVLVIFTNKDNKSDKPNDKLISETAKS VMKEF" misc_feature 15874..15945 /gene="blaZ" /locus_tag="pSAS19" /note="Signal peptide predicted for pSAS.19 by SignalP 2.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.999 between residues 24 and 25" misc_feature 15943..16716 /gene="blaZ" /locus_tag="pSAS19" /note="Pfam match to entry PF00144 beta-lactamase, Beta-lactamase, score 376.1, E-value 2.2e-110" misc_feature 16048..16095 /gene="blaZ" /locus_tag="pSAS19" /note="PS00146 Beta-lactamase class-A active site." gene 17088..17621 /gene="bin" /locus_tag="pSAS20" /note="synonym: sin" /pseudo /db_xref="GeneID:2853419" misc_feature 17088..17621 /gene="bin" /locus_tag="pSAS20" /note="Similar to Staphylococcus aureus Tn552 transposable element potential DNA-invertase Bin3 SW:BIN3_STAAU (P20384) (202 aa) fasta scores: E(): 6.3e-33, 80.09% id in 201 aa, and to Staphylococcus epidermidis plasmid pST6 recombinase Sin TR:Q93SQ8 (EMBL:AY028779) (202 aa) fasta scores: E(): 1.5e-32, 78.71% id in 202 aa. CDS contains an internal deletion relative to orthologues;potential DNA-invertase (fragment)" /pseudo /db_xref="PSEUDO:CAG43273.1" misc_feature 17088..17447 /gene="bin" /locus_tag="pSAS20" /note="Pfam match to entry PF00239 resolvase, Resolvase, N terminal domain, score 165.5, E-value 5.5e-47" /pseudo misc_feature 17100..17126 /gene="bin" /locus_tag="pSAS20" /note="PS00397 Site-specific recombinases active site." /pseudo misc_feature 17451..17609 /gene="bin" /locus_tag="pSAS20" /note="Pfam match to entry PF02796 HTH_7, Helix-turn-helix domain of resolvase, score 86.7, E-value 2.9e-23" /pseudo gene 17861..18010 /locus_tag="pSAS21" /db_xref="GeneID:2853418" CDS 17861..18010 /locus_tag="pSAS21" /note="No significant database matches. Similar to Borrelia burgdorferi conserved hypothetical protein BBT02 TR:Q9S019 (EMBL:AE001583) (117 aa) fasta scores: E(): 1.7, 40.9% id in 44 aa" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_031713.1" /db_xref="UniProtKB/TrEMBL:Q6G5U6" /db_xref="GI:49398114" /db_xref="GeneID:2853418" /translation="MSYRCLMIVNLRIIELILYIDEQNKQQSRKNTFRLFLLILYFIY FCYNK" misc_feature 17951..18004 /locus_tag="pSAS21" /note="1 probable transmembrane helix predicted for pSAS.21 by TMHMM2.0 at aa 31-48" gene 18035..18328 /locus_tag="pSAS22" /db_xref="GeneID:2853405" CDS 18035..18328 /locus_tag="pSAS22" /note="Similar to Lactococcus lactis lactococcin 972 precursor LclA TR:O86283 (EMBL:AJ002203) (91 aa) fasta scores: E(): 0.021, 30.76% id in 78 aa. Identical to Staphylococcus aureus hypothetical protein SAP019 TR:Q9AC72 (EMBL:AP003139) (97 aa) fasta scores: E(): 4.5e-39, 100% id in 97 aa" /codon_start=1 /transl_table=11 /product="putative bacteriocin precursor" /protein_id="YP_031714.1" /db_xref="InterPro:IPR006540" /db_xref="UniProtKB/TrEMBL:Q6G5U5" /db_xref="GI:49398115" /db_xref="GeneID:2853405" /translation="MKKKFVSSCIASTILFGTLLGVTYKAEAATVHVAGGVWSHGIGK HYVWSYYSHNKRNHGSTAVGKYSSFSGVARPGVQSKASAPKAWGGNKTFYSLH" misc_feature 18035..18118 /locus_tag="pSAS22" /note="Signal peptide predicted for pSAS.22 by SignalP 2.0 HMM (Signal peptide probabilty 1.000) with cleavage site probability 0.966 between residues 28 and 29" gene 18403..20337 /locus_tag="pSAS23" /db_xref="GeneID:2853426" CDS 18403..20337 /locus_tag="pSAS23" /note="Similar to Lactococcus lactis hypothetical lactococcin 972 immunity protein LclB TR:Q9L651 (EMBL:AF242367) (648 aa) fasta scores: E(): 1.7e-13, 21.9% id in 662 aa. Almost identical to Staphylococcus aureus hypothetical protein SAP020 TR:Q9AC71 (EMBL:AP003139) (644 aa) fasta scores: E(): 0, 99.53% id in 644 aa" /codon_start=1 /transl_table=11 /product="hypothetical bacteriocin immunity protein" /protein_id="YP_031715.1" /db_xref="InterPro:IPR006541" /db_xref="UniProtKB/TrEMBL:Q6G5U4" /db_xref="GI:49398116" /db_xref="GeneID:2853426" /translation="MKKFKLFLDFSTLLLISGLLFLFFFKENEEIIPESSNILTISNW DKSNSKSKVLDVIESGAKNQNIQIIKSVKDFDNKKEFFVFNSKRNNSDFIRNKTSLLT PSDLLNREIKGKYYIIGEHFNVEELTSELKKAGLTSEVEYINRNILFFELVIDNDLLV PFIFLGVLYLLYFLYDRGYNLKFYAIQKLHGFSTTRIIFHNIHIKIIYWLVLTAIFFI ANILIIHIANLELDFLMFIRRFIVLLFVFACITTLLWLISYSLLLKLSIPLILKGKKG YKFSIFMGTFTKCIMVLILSFLLAQNLNAYTKLKNIYDSEKIWQKLDNYYTLEISPNI RNSEEKQILETNIYNLIKKSEQLGGLLLKNNNIANPDKNNYIPENGNVFFINNNFLNF YKNINHDFKMIDNHSDKINVFIPPRLQYQKSEIQKNHQGWIDFQKQQNKKVDVQVLSK NVSVFSFDRTTNLKFGYLDSPIVMLLTLDDLTGDFYLAAVSQGGYLFKDLDTLKQLLK DNHLEEEISGITNYKDSVLNELNETKTKMIITIVTIIINVFILLIATVFETIQYFSWN KKQLLLRKIHGYSLFSSNVRYLTISILLSIMLAYTTHILFGSKILLFIIMSIAIIQHL LQIAYIKYLEKYFKDLMREI" misc_feature order(18421..18477,18874..18927,19012..19080,19123..19191, 19228..19296,20014..20073,20134..20202,20230..20298) /locus_tag="pSAS23" /note="8 probable transmembrane helices predicted for pSAS.23 by TMHMM2.0 at aa 7-25, 158-175, 204-226, 241-263, 276-298, 538-557, 578-600 and 610-632" gene 20341..20646 /locus_tag="pSAS24" /db_xref="GeneID:2853427" CDS 20341..20646 /locus_tag="pSAS24" /note="Similar to Bacillus subtilis hypothetical protein yxea precursor YxeA or bsu39620 SWALL:YXEA_BACSU (SWALL:P54940) (115 aa) fasta scores: E(): 0.012, 30.69% id in 101 aa" /codon_start=1 /transl_table=11 /product="hypothetical protein" /protein_id="YP_031716.1" /db_xref="InterPro:IPR006542" /db_xref="UniProtKB/TrEMBL:Q6G5U3" /db_xref="GI:49398117" /db_xref="GeneID:2853427" /translation="MKRTLIIVTIVLLIIILSLLLVRNEHLDRFNPLLKEKVSYAKVP KDTQDYRNITVYSKNGEKKSYKLDFLGYDPTKEYVKVNHKGKYVRSIEYITKIFLAF" misc_feature 20341..20412 /locus_tag="pSAS24" /note="Signal peptide predicted for pSAS.24 by SignalP 2.0 HMM (Signal peptide probabilty 0.953) with cleavage site probability 0.581 between residues 24 and 25" ORIGIN 1 ggtgaccgat agtgataaaa cttgaaaatg tttttgtaaa aaaaggtaat aaaaacattt 61 tagacggatg taactttaat tttgaaaaag gaaaatcata tgctttagtt ggagaaagtg 121 gcgctggaaa atctacttta cttaacataa tcgctgggtt cgaagatgta tcacaaggaa 181 gcatatatat tgaagataag ttactaaaaa agaaagtcga tttttataga tatacgttag 241 ggtatttgtt tcaaaatttt gctttacttg aaaatcaaac aatcagtcaa aatttagatt 301 tagccctaaa gttcaaaaag aataaaaaag ataatatgaa cttaaagaaa gaagtattaa 361 aaaaggtcgg cctagattta gatatcaaga gaattgttag ttctctaagc ggtggtgaac 421 aacaaagagt agcattggca agattaatat taaaagatcc gaaaatcatt ttagctgatg 481 aacctactgg gtcactagat acgaaaaatg gaaaaatagt tattgattta ttattgaagc 541 tacttgatga aaataaaact atgattgtcg taacgcatga tttagagctt gcgaaacgat 601 ttgatgtcat agtcaatatt tctgagctta gaagttaaga aatattgata ggtatagcca 661 tacatagaga aaaaacaata tgaaatatga ttagtcatta cttttgagtt ttttaattta 721 aggactggat tttccagtcc ttttttatga tgataaaaaa caagcctcgc agagcacacg 781 tttttaatga cttatttggt ttttaataag tctagtatgt taaaactttt ctgtgtttag 841 gtgttataat catatgcagt ggaagttgat ttattatgtc ttattcaatt attcgagttg 901 ttaaagtgaa atcaaaaaca aatacaagag gtattcaaag acatattcaa agagaaaata 961 aaaattatga aaatatagat attgatttaa gtaagtcata tttaaactat gatttagtca 1021 atgatactaa gtttgatttt aataaaaaaa ttgatgagaa aatcgaaaaa aattataaag 1081 ggaaacgcaa aattagaaca gatgccatta aacatattga tggattaatt acatcagata 1141 atgtgttttt taatcaatta tctgaagaag agacaaaata gttttttaag catgctaaag 1201 cgttcttgga agaagaatac ggtaaagaaa atcttttata tgcgacagta catatggatg 1261 agataacacc acatatgcat tatggcgtag ttcctattac taaagatgga cgtttaagtg 1321 cgaaagaagt tgtaggtaat aaaaaagcat taaccgaatt ccaagataga ttcaatacgt 1381 atattaataa gcaaggctat gatttaaaac gaggaatttc tagacaactt acaaaagaaa 1441 aacatgatca agttagccgt tataaacaaa aaacagaata tcataaacaa atgcatatgc 1501 gctagaagca aatagaggat tatttaaagc aagaaaatga taaattgata caaaactacc 1561 aaaaatcatt agatacgctt aaaaagcttc tgagtgtcat gtatgaaatt aaaactaaaa 1621 atgtaggtgg atggtttcat aaagagaagc aagaaacagg caatattgta attactaaaa 1681 ctgattttga aaaatacact aaacaaatta aagcagcaca gatgatatta gatgattatg 1741 aatgtattaa aagtgggaag tcattaaaaa aagcagagaa acaaaatgaa agtttagtta 1801 atgaattgac tagtgtccat atggaaaatg aaaagctggt tgaagaattt aatgatttag 1861 cacaacgtta taattatttg ttgtcagaga atgaaaagaa agataaagaa ctaaattata 1921 cgcttaagtt attcaatcaa gtttttaaaa tcattaaaag tatgatgaaa gaagagcgtt 1981 atcatacact aataaatcat attgataatc atttagacaa ttctaaaata cgtgaagtaa 2041 tgacgataga taacaatgat gaacagttct ttaagaagaa atatcaagca caagaacgag 2101 aaatcatttt taaagaagat agagaagatg gttatacatt ataaaaatag atatttaaat 2161 gcatttgttt gaggttaatt attttttagg atgaaaaata ggtagccccc tcgaactttt 2221 taatatattt ttataaatct ttttaaaaac ctttttaggg gcttgttcag ccctagagca 2281 acaagggatt tcaatgccta aaggagtgtt tttgccacat aaaaggagtg tttttgccac 2341 ataaaaggag tgtttttgcc acataaaagg agtgtttttg ccacataaaa ggagtgtttt 2401 aaaaagttgt actcctttta tggtttgaca ttgataataa agtagtttat tgtgaataaa 2461 agttttcaaa tgggagtgta tattcaaacg atactccttt aggttcgtaa taaagggggc 2521 tgtatcatga caggagagac agttgtatat aaaaatgaaa tgaatttagt tccattaagg 2581 cgatttacag ctactgaaat taatttattt tttgcgatgt gtaataaatt aaaagagcaa 2641 gatactaata cattacgttt gtcttttgat gaattaaaaa aattaagtaa ttatagtcca 2701 gaaacacgta atattaatag atttgctaat gatttagata atgtgtataa aaagatgttg 2761 aatttaacca ttagatatga agatgatgat gtaatagaaa gattcgtttt attcaatcat 2821 tatagaatcc ataagcgaga acaatatcta gaaatatcaa cttcttcaaa tttaaaacac 2881 atattgaatt caattactaa taattttaca aaatttgaat taaaagaaat gactaggctt 2941 aaatctacat attctaaaaa tatgtttaga ttacttaaac aatacaaaca tacaggctat 3001 ttaaaaattc acatcgatga ttttaaaaat cgtttagata tacctaaatc ttatcgtatg 3061 actgatatta acaaaaatgt ttttaaacct attattattg aattaggttc tatatttaat 3121 aatcttacta tcaataaaat caaagcgaaa aaagggcgta aaattgaatg gatagagttc 3181 acatttgacg ctgagaaacg cattcataac aaacgacaac ctcaaatgtc caagatagat 3241 aaatcaagac aatatgtgag gcgtgaaaag acgcctaaat ggttagaaga acggtcatat 3301 gaaaagcaac ctcaaaaaga ttacgaccca caattagaga aagaacgaga ggatttttta 3361 aaacaacttg aactgaattg ggaataacgc aacttttcgt tgcgagttgg ttaactaaaa 3421 gtatcgtgac ttttcaatta ttttaattat agtttgagtt aaggagtgat tttatgaaat 3481 tagctgaagc tataaaagaa cagagagaat taaaaggatg gtctcaagag gaattagcca 3541 atatattaaa ggtttccaga caaagtgttt ctaaatggga aagtgccaaa aactatcctt 3601 ccttagatat tttgattgct atgagcgatt tattcggaat ctctttagag catttaatta 3661 aaggagatgc acgttttaaa aaagttattt tagagggaaa ttatagagag agtaacaggg 3721 taccttccat agttgatttt ctttatgatt tttggtggct tttctttcct gttgccggat 3781 ttttagtttg ggccattcat tcgttcatgg gttgatttta aaatcaaaat ttaatgcaca 3841 tcaggcaata tgaaaaagga aagtattttt acgacacctt gaaatgtatt gataagaata 3901 gaagagttta caaaaggcaa tatgaaatat atactaaaag taacaagaat ttcgtttgta 3961 aacgcaccaa accccgaact attcgccgta gttcggggtt ttttggtgaa ttggctttaa 4021 tcgtctatat tattttttat cacgtttatg gagctaataa ccaaataacg taacgagaca 4081 accactgatt gccgttgtca tgatgtgaac aagaatttca ttcataaatc gcacctcctc 4141 tctacgccga attgacatct gagagataga cgaccttcac attataacaa atctctcctt 4201 ccatgaaata acatgaatat taataaatgt tgttatatat taaagataaa taaatataag 4261 ggtgggattg ataatgtttg gcgatcaaag acaagaagct acaaaatatg tcattaaaga 4321 aggttatcaa gatatttatt tcctcaacaa aaacggggaa tggtattatt tcgaagtaag 4381 aagtgtctgg cgtggcaaac atattattag agttaaagat ggtttgttag gttggagaaa 4441 agaaatagta actgaataaa gatacaaaat acttgccaat gcaaaagaat gaaaacgcgt 4501 caaatcaacg atgtataaaa gatgcataaa agataatgaa agtaaaaagc cataaatgcc 4561 gtcatatagg catttatggc tttttgtttg gttaatataa tcgatgatta tcagaagttc 4621 aattcacata aaaaagcacg gctaatttat gaacttgtcg taccttttta attaaaatta 4681 gttaccaaat agacgactcc aaaacccctt tttagcttcc gtttcttcac tcttttcttc 4741 tatatcacta tcattgtcat ttggattagc tgaaacgtct tctctttgtg cttcttcacc 4801 ttgtgtactt tcgtcgcttt gacttccaga tatatcctta tattgaatct ctgtttcttt 4861 agtttcttcc tgatctcggt taatatttac aggactagta gtatatgttt tttcttgtgc 4921 atcaacgttt tgtctagcgt tagtagatgt atcaaaggga taattaaggt gtttttcttt 4981 ttctaattga ttttctaatt tttgaatctt tttattactc tctaatgcca gaacttgctg 5041 attctcaagc aattttgatc tttcttgcaa ctcttttctg taaaattcaa ttgttgtttc 5101 atgacgactt tcttgttttt caatttgatt atttaaagta ttaatctgat ttttaagtat 5161 ttctactatt tgagcatcgc ttttagattc aaaattaata ttatccgact cttttttttg 5221 tttgtcttca gtagcacttt caaaaccata tttcttttta ttttcattta ctctttgaat 5281 gattttttct atatcggtat cttctttaat aaacgaagtg ttttcctgtt ttattgtctc 5341 tatattcaat ctcttaatat tattaaaaat cgtttgctta ctaaccccta attcttcact 5401 caaccttttt acacttttca tatcatcaac cctttataaa atgtttataa acccttagta 5461 aaatattata agcttataac gattaataat aaagtaatcg acaaataaaa atataaattc 5521 gccaaccttt ttatcaaggg tttgtaaagg gtttatggtg tttatcaaag gtttataaac 5581 ctatattttc ccttttttaa gtttattcaa agtgctataa taaccttaat ataacaaaaa 5641 ccccaactac tggtaatagt cggggtttgg taataaaacg tttagaaact gtttatttat 5701 ataattttcg atacttagat tatataatga ataatatctt agtgcaagca cctagattat 5761 taaataaaca caaaaaatgt aagcgtttta ttacctctta ccaaactaaa attcggaaag 5821 aggtttttat tatgtcaatt aaactttaca acataaatga ggaatacaga gaaaagtttt 5881 atcaaatacc gaaagtattt tttacaaatc ctaaatatat caagttatcc aatgatgcaa 5941 aaatggcatg gggtattctt agagatcgtt tagatttatc aattaaaaat ggttgggttg 6001 attctaaaac aggtaatatt tatttttatt acaaaaatga aaacccacaa aatatattaa 6061 attgtaaaaa agataaagta attaaaataa agaaagaact taattgaagc cgaattacta 6121 ctacaagaaa gacaaggttt aaataaaaca aataaacttt atttagttaa accagaagta 6181 acagaagatg atatttataa aattcaaaag caagaaaaca ctgttgagag cagcaatggc 6241 aaggaagtcg gaaaaaccga cttcaagaag tcgataaaac agaatctaat gatactgaac 6301 ttagctatac tgaatctaac aatactgaaa taaatgatat gaatgacttg aatgatatag 6361 agtccgaaaa tgaaattagt aatcattcaa gtcaatgcaa ggttatttaa tgcaaacaat 6421 tttaactgaa tttgaagaaa cacatgcact ttatatgcgc cgtaaaaaca tgaaacaata 6481 taatatcttt aatcaatcat tcaaatgatt ttataacaat caaagataca tcaaaatatt 6541 tcgttaaatc atctgacttt ttaactgata gcgatgaaaa aatcaagaaa ttgtcagtga 6601 tctagaaaaa gatttgtatc gaaaacgtat gttaagttat ggtggattgc ttaaataaaa 6661 acataaaatt ttaaacttag acgatgctga agatggcaat ttaattaata caagtgatga 6721 agataaaata gcaaatgaag aagaaaaggc acattcgatt acggcaattt tgaaaagcaa 6781 aattattatt taaggcatta gtgataactg atgtcttttt ttattacttt tttgtaaaaa 6841 aagtgctgtc ttatttttgt gacaaatgct gtatgtagtg tcacaaaagt gtgacacttg 6901 agctttttat gaccccacac aaaaacattt tcgaagcctt gaaatatctg gctttgccag 6961 atcccccatt cattgatggg gacaaatttc ccttatgctc ttacggagat tttagagtta 7021 aattaaaatt tctaagtttt tgataagaaa agccctgcag gaatttagaa gaacgtgggg 7081 aagttttaaa gaattttatg ctaattttta atttgcatgt aaatgggcag tgtctaaaaa 7141 atagttattg tttttgctca aatttttgtt tttgaaaata gcatatattt atttggctca 7201 tatttgcgtt ttaaaagcgt atataagttt tagataaaac tatatgaatc acccctaaat 7261 ctttaaaatg ccctcttaaa attcaaaata agggcataaa aaataaaagc ccccccagat 7321 aaagtgaggt agctttttct ttcgacgccg tatcgtcgat gtaattcaaa aaagttatgg 7381 gctataaatc tacatcactt tttcaagaat gtagtagcaa tattcaacgc aaattaattt 7441 ttataactgc tcggaatatc tcaagccgtt tctttaattt tgaaataaaa aaatcgacga 7501 aggtcgatta cgtttttgca catgtcgatt tatccgacgt ataagtgcgc ccttacggga 7561 tttaactaga ttatgacgac gaattttaga cctgtaaagc aataacaatt aggtctacca 7621 ggtagataaa gtgtacaaag tagaaccgaa aaagttattt attttgaaga tttttcgtag 7681 atataatttg ctaataaact tcctaaaact gaataaagaa aaatagttaa actcatgtaa 7741 tcactccata attctgaagt gtctccatgt ctacgcatag acacgcattt atgccgagaa 7801 aatttattgt gcgttgagaa gaacccttaa ctaaacttgc agacgaatgt cggcatagcg 7861 tgagctatta agccgaccat tcgacaagtt ttgggattgt taagggttcc gaggctcaac 7921 gtcaataaag caattggaat aaagcaccta tgattattta aaaatataag ttataataca 7981 ttcaaaaata tttttgaatg aggtgtatta tgattcaaac ggttgttgct gctgctgttc 8041 tttatattgc tacagcagtt gatttattag taattttatt aatatttttt gctagagcaa 8101 agactagaaa agaatataga aatatttatg ttggtcaata tttagggtct attattttaa 8161 tattagttag tttgttttta gcttttgtat taaattatgt tccagaaaag tggatattag 8221 gtttattggg tttaatacca atttatcttg gaattaaagt ggctatttat gatgattgtg 8281 aaggagaaaa gagagctaaa aaagaattga atgaaaaagg attgtctaaa ttagttggta 8341 cggttgcaat tgttacgata gcaagttgtg gcgccgataa tattggttta tttgttcctt 8401 attttgtgac attaagtgtt actaatttat tacttactct gtttgtcttt ttaattttaa 8461 ttttcttctt ggtgtttact gcacaaaaat tagctaatat cccaggaatt ggagaaattg 8521 ttgagaaatt tagtcgttgg attatggcta tcatttatat agctttaggt ttatttatta 8581 ttattgaaaa tgacactatt caaacaattt taggatttat attttaattt agggtgtgat 8641 tttatatgag ttatgaaaat acttgtgatg tgatctgtgt acatgaggat aaagttaaca 8701 atgctttaag ttttttagaa gatgataaat ctaagaaatt acttaacatt ttagaaaaaa 8761 tttgtgatga gaagaaattg aaaatcatat tatctttgat taaagaagat gagttgtgtg 8821 tttgtgatat ttctttgata ttgaaaatga gtgttgcttc aacttcacat catttaaggc 8881 ttttatataa aaatgaggta cttgattttt ataaagatgg aaagatggca tattatttta 8941 ttaaagacga tgaaataaga gagtttttct ctaaaaatca ggagggtttt tgaaacgagt 9001 gaaacgagtt tcttcttgtc ttgatactat aaaaaacaaa gttatataac ataagacgac 9061 ttatcggaag ttatctatca tcgtatattc ggagatgata taatatacag gtcgcctatc 9121 tctcaggcgt caattcagac gcagagagga ggtgcattac tttgctaatc attttcgttc 9181 acatcatagc accagtcatc agtggctgtg ctgttgcgta ttatacttat tggcttagta 9241 aacgcaacaa ataattaata ggcgacttta taagccagca tcgaaaaaat ccccacacta 9301 ttgcagtagt gtggggattg gtgcattaat tgttaatcat tttcgttatc tagattataa 9361 cactggttag tagaggaatg caaaaagata atattttaag tagtcttttt ggatattatc 9421 atatgatata tttatgtaaa ttgatttttt attaaaggag catatatgag taatactaaa 9481 attaaatatc aaatttttat ttcatcaact tatttagatt taatagaaga gcgtcaagca 9541 gtagtagaag cagttttaaa cagtaataat attccagcag gtatggaact ttttaactca 9601 agtagtaaaa gccaatggga aataataaca caatggatca atgaatcaga tgcagtgata 9661 ttattgttag gttctagtta tggaacaatt gatagtgaag aaaatattag ctatacagaa 9721 atggagtaca attttgctaa atctttaggt aaacctatat atgtttttga gatgaataaa 9781 gcatctaccg aatccgcgga aaagtctaaa aaattagatg agtttaagac aaaagttaaa 9841 gaaaatagac taattagtcc aaatattaat gatatttcgg aattaaaaag taaagtatct 9901 cagactttaa tacaaagtgt acctagtgaa attaaagaaa atagtggatg gataagagta 9961 caggattgtg gtaaaaatgt aaaaaaaata aaaaaattgg atgctataca aagagattta 10021 gatacatatg attttttgaa agaagaactt tataaaatat tcatgtatgt aaaaactttt 10081 tatttccagc acacaccatt tgatgatagt tatattgaag atatagaaaa ctacctgcta 10141 aattatgaaa ataatccaca taaatcattc atgaaaataa aagtttatga ttataaaaac 10201 tttttaaaac atttaaaaaa catattgatt ttaattaaac aaggaaaaaa gccattagct 10261 caagccggtc attttaaatt aagcttttac gaagaggatt taaaattgtt acaaaaaaat 10321 tataaggcta cgctagtcga tattaaagga ttgaataaac atttttataa ggaatgtgat 10381 aggtgcttaa aagcatataa tatttttatt gagggtataa ataaacattt atatgaatga 10441 tataagtaca ccctaatcat acatatataa ttgttgtcac tttactcgat atatgagaat 10501 gactttacct aataaatgag aaaagcaacg cactatatta aaatgcgttg ctttttctat 10561 tatcgaatac ctataactaa actatctcta gctgtttcta caacttctgt acttatttta 10621 tctaaaccat ttatatccat aattctgtct atctgcgcaa ataaacgatg aatcagccta 10681 aaattccctt ttgtaatttt aataatcgtc gttattgcct cataatcagt aaagtcttcg 10741 agttttaaat caaatcctaa gtcttgacac ttatattcta atatatgatg cgtctcatct 10801 ttacttagat tatcaaattc atgcgcaaaa cctatacgcg aatatagttg aggataacga 10861 gatagttttt tctctatacc cggcatacca ataaatatca ttgctaaatt atgttcatca 10921 taaatagctc ttaattgctc taaatgttgc actttaaggc gatcaatctc atctactata 10981 attaaatcaa tatatttaaa catatcacta taatgtttac tatgttcatg tccatactta 11041 tttacaacat gtaatttttg acccaaatca atgattgcag taatactata taaatcattc 11101 cttaatttag tttgtttttc tgctggtgca ttataaaata ttttatccac cgatagtatg 11161 tcatcggtcg ttttactagc taaatcttcc caacctctat ggttaacttg tttttcaata 11221 gtatcccaat ttgtgtaata cctagacgat aaagtttttc cgacaccagg ttgaccataa 11281 catataccaa tatactgata tttaatacaa gcatcacaaa attcagcaaa gcgtttatac 11341 tctttcgttt caatgaaatt ttgattttta gtcattacga tatctcttta attttttgac 11401 gtttttagtc gtttcttgag gtgaataacc acaatttttt tcttccttaa ttaattccgt 11461 ggtacttgga ctagcaatat tttgttttaa gtgttttctt ctctgactac gagcgtgctg 11521 tatgtctttt atatctatgg agtaatcagc tatttctgga catatagcag tacaaagaaa 11581 ttcatctcta taaaacacac gtatttcagc catatcattt gggttataac gaatcagtac 11641 atattcacct acataagctt ctagattagt attactgtat cgaaaacctt gaaaatgaat 11701 gccatctgaa tgaacttttc tagatttagg tatttccaac aataataaat caagttgttc 11761 taagctactt ggcatattag ggaaaaagtg attactattc caatgactga taggcgtggt 11821 atgaatagta ctatgttctt tttgattata atcttcaatt aaaaagtaac gtaatttttc 11881 ttcaaaattg tgaaaattta ttagattact aggagtatca ttattgttta tatatccagg 11941 cagttgttct aagaaagttt gattaactgt ctgaaagaat cgttctattt ttccacgacc 12001 acgaggaaca ccaactttcg aaaacatcaa gttaattttc aaatcaatag cgacttgttc 12061 catatgatgt gaagtaaagt cacttccatg atctgtgtag aatttttcgg gtataccaca 12121 cactggccaa ttggtatcat ttttattcca tattgcttgg tgtagtgtta atgctgtgtt 12181 ttgagcattg gggggcatca aaacttaaga aatagcctgc aattgcacga ctatagtcat 12241 ccataataat agttagccat ggtctattga tattaccttt ttggtctaat ataaagatat 12301 ctagcaaagt atggtctgct tgccatattt cattgggtct cgaggattct ctaatttgta 12361 ttaaatcgta cttattttga tagtattttt caccctgatg agagaaatca ataacagact 12421 taggcatcgc tttaataaca ctataaattt gtttataact tggtatatca aaattatttt 12481 ctttacagta attagtgatt ttcctatgaa ttgtggctat actatttctt ttattcataa 12541 gcgcaaggcg ttcaatttca tcaattatct ttggttctat tttacgtgtt cctttatcag 12601 ctcttgtcgc atagattagt cctttaagtc cttgttcttt atatgactta ttccacctat 12661 aaagggtgct taacgcaatc cctttacttt tagaaatact tgataaagat tgttcgccta 12721 ggataaaagg ttttataata ttgtactttt gagtagcttt tttacgtttg gcttcagaaa 12781 aattagttag atacttctcc ttgtttttca tcttccttgt cctccaattt attgaggtaa 12841 cgataaattg ttgtacgtga aacatgccac tgttctgcaa ttgtcttaat tggcgtgcca 12901 ttatcataaa gggtcttaag taagtttaaa tctttttgat ttaatttttc tggtctgcct 12961 ccatatcggc ctctagcacg tgctgcaatt cggccagctg aagaacgctc taaaattaaa 13021 ttacgttcaa attctgcaaa tgctgcgaat aaatgaaata ataattgtcc agttgaactt 13081 gatttatcca ttgttatatt ttcttctaaa ctatggaaac ttacacctcg ttcgttaagc 13141 tcattaacta atgtgattaa atcctccata ttacgtccca atctatctaa tctccaaaca 13201 acaatcgtat ctcctgacct cgcaaattca attgctttat ctaaaccagg tcttttactt 13261 tttgaaccac tcatatggtc attaaatatc ttttcacaac catatgtatt taatcgatcc 13321 tcctgtaaat ttaaattttg taatccagtt gaaactcttg cataacctat tttcaaaata 13381 caaacctcct atatacatct tcagtgtacc ataacttaat tacaaggtct tttatgaaca 13441 tagatttttt gttgggtttt tgaacacata atcctatgta attcttactt atttagacat 13501 aaaagaagag tgtacagaaa atggttattt tctgtacact ctcatcttta ataatatatt 13561 ttaaatgtca ttaacaaaac taatttaata agagtcaagc atagtttaca tatgtattat 13621 gtttgaaaca tacctctaat tactttttac taatatcatt taaaatatct cgcaattctt 13681 caatttcttt gttatttaat tcttcatttt tcgcaaaatt caacactaaa cttttcatat 13741 cccctccata cagtttatta agaaaggttt tagcagtttt cattttaata tcgtcttctt 13801 taatatttga tgagtaaaaa taaatatttt ctgatttgta tcgttttata atctcttttt 13861 tatatagtct tgtgattaat gttctaatcg ttttatcact aacttcttta tatttttgaa 13921 tttcaactac aatttcatta gctgatactg attttttatc ccatattata ttcataacat 13981 cccattcagc catagatatt tcaacttgct tattggccat ttaaaacacc cattcctttt 14041 aatatttttt cacttattaa ttcagcattt ttcccagacg ggtttccatc tgataaatgt 14101 gtagcaaaat aatacttatc atgatttgta attacgtaac ctacaaacca cccattatta 14161 tacttcccgt ttactatacc tgtacctgtt ttcccataca gttcatactt ttcatttttc 14221 ttaatcaata atgaagaaga taattgattc tttgcttttt tactaaaatg gttattttgt 14281 tccatcatat ttttaaaaac tattacttgt tcaagattag atattttcaa actatcttcc 14341 atccaatagc ttttataact tcccaaattt tcattaccat aatttaattg cttgagttga 14401 gccgcagtat agttctttgg tatttgatta ctaatacgtt cgaagtacca attaactgaa 14461 ttttgcattg ctgtatttaa atcttgttcc ttattccaag actcgaaaag ataatgttta 14521 tgattccatg acatacgtga atttttatca cttataatat gacggtcaag tccaaacagt 14581 gctaaataaa ttttgtaagt tgaatcagga gaatatcttt ttctactttc tttttcatta 14641 taaatatagt atttgtcttt cttcatgctg tacataacga aagatccact atttgaacca 14701 aaatttttac tttcatctaa aatttggtaa tcgctttgta agggcttttt ataattataa 14761 tcagttaagg attgccccat taaaaactgg ctttgaatta tcatgataaa aaaagtaaaa 14821 atacaaataa aaattagaat taattttgac tgctttttca gatcggcttc ttttatatta 14881 attaatctat ttttgagtaa tgatttttta ccattaaatg aatggcttaa tatatttttg 14941 ttgaaaaatg aggtttttaa tacggagtcc ataatagatt ccgcataagt tttaaattca 15001 tttttgttaa tattattaag gacaaatcta tcggcttcta cttcattgtc atgaattatc 15061 tttcttttta caatatgtac taatggattg taactcataa taatagagaa gatattaaag 15121 ataattaaat gtaaagtatc tctattttta gcatgagcat attcatgcag tattatatat 15181 tttagtcttt tatcaattac acttttaaaa tatgaactag gaatcaaaat aatatatttc 15241 ccataccaaa aagttattgg agattgaata gcctctgctt ttcgaatcac aatatttttt 15301 ttatattgat ggttgaaaag tatcgtatct attttatttt tttcattttc gtttagataa 15361 agtgactgtt tctttaaata tttaagatat aataaggctt tcaaaaattt aaaacttaaa 15421 ataataacta aaactatcca aataactgtg cagatattat caattgaatc ccaattaaac 15481 ttatggatat ctgttgcgaa ctcttgaaca ggtttggtgg taattatgtt atggttcaag 15541 tcgtgtgact tactttcaac tgtgggcgct tgattattca cattattaaa ttttataaaa 15601 gagaatttaa ttgggataaa aggaattaat ccagcaagca cagttagata ccaaacttta 15661 taatttaaac tataattaaa atagcgtttt aatatgtacc taaaaaagac taacaataga 15721 aatataaaac aaaagcttac tacactcatt attagtaatt tagccatttt gacaccttct 15781 ttcaaatatt tataataaac aattgacatc aatattacaa ttgtaatatt attgttttgt 15841 aaaaattaca actgtaatat cggagggttt attttgaaaa agttaatatt tttaattgta 15901 atggctttag ttttaagtgc atgtaattca aacagttcac atgccaaaga gttaaatgat 15961 ttagaaaaaa aatataatgc tcatattggt gtttatgctt tagatactaa aagtggtaag 16021 gaagtaaaat ttaattcaga taagagattt gcctatgctt cgacttcaaa agcgataaat 16081 agtgctattt tgttagaaca agtaccttat aataagttaa ataaaaaagt acatattaac 16141 aaagatgata tagttgctta ttctcctatt ttagaaaaat atgtaggaaa agacatcact 16201 ttaaaagaac ttattgaggc ttcaatgaca tatagtgata atacagcaaa caataaaatt 16261 ataaaagaaa tcggtggaat caaaaaagtt aaacaacgtc taaaagaact aggagataaa 16321 gtaacaaatc cagttagata tgagatagaa ttaaattact attcgccaaa gagcaaaaaa 16381 gatacttcaa cgcctgctgc tttcggcaag actttaaata aacttatcgc aaatggaaaa 16441 ttaagcaaaa aaaataaaaa tttcttactt gatttaatgt ttaataataa aaacggagac 16501 actttaatta aagatggtgt tccaaaagac tataaggttg ctgataaaag tggtcaagca 16561 ataacatatg cttctagaaa tgatgttgct tttgtttatc ctaagggcca atctgaacct 16621 attgttttag tcatttttac gaataaagac aataaaagtg ataagccaaa tgataagttg 16681 ataagtgaaa ccgccaagag tgtaatgaag gaattttaat attctaaatg cataataaat 16741 actgataaca tcttatagtt tgtattatat tttctattat cgttgacatg tataattttg 16801 atatcaaaac ctgattttcc ctttattatt ttcgagattt attttcttaa ttctctttaa 16861 caaactagaa atattgtata tacaaaaaat cataaataat agatgaatag tttaattata 16921 ggtgttcatc aatcgaaaaa gcaacgtatc ttatttaaag tgcgttgctt ttttctcatt 16981 tataaggtta aataattctc atatatcaag caaagtgaca taattgttga atttatagta 17041 actgaaacgt atgattaggg tgtatattaa ttatgagatg gaaatcaatg attgttggct 17101 atgcgagagt atcatcgaac gatcaaaatt tagaaagaca acttgaaaat ttaaagacgt 17161 tttgtgctga aaaaatattt acagaaaaac agtctgggaa atctattgaa aatagaccag 17221 tacttcaaga agcacttaac tttttgagga tgggagatcg gtttgtagta gaatccattg 17281 attgtttagg tcgtaattat gatgaagtgg ttggcaatcc attactggat aagtttatga 17341 aagatttaat tattcaaata ttagcaatgg tttcagaaca agagagaaat gaaagtaaac 17401 gtcgacaagc gcaaggaatt caagttgcga aggaaaaagg cgtatataaa gggcgcccct 17461 tactttattc accaaatgct aaagatccac agaaacgtat tatttatcat cgcgttgtag 17521 aaatgttaga agaaggccaa tcaattagta aaattgcgaa agaagtaaat ataacaaggc 17581 agacgattta cagaattaaa aacgataatg atttgattta ataaactttg tttcaatgca 17641 actgagagct tctcacagcg tctcaaaaca gatgattata atctattatg agtttttaat 17701 aataagacaa gaaaagagag cgtaaatatg ctctctcttt ttctcattaa cgaacgttta 17761 taacgcaatc atgatgagag tattaataca gttaattatt tgaaatactt tagtttatat 17821 aatgttgaat tataggtagt gaaattgtat ttattataca ttgtcatatc gttgtttaat 17881 gatagttaat ttaaggataa tagaattaat attatatata gatgaacaaa acaagcaaca 17941 gagcagaaaa aacactttta gattattttt actcattctc tattttattt atttttgcta 18001 taataaataa ataatattaa atggaggtgt ttttatgaaa aagaaatttg tttctagttg 18061 tattgctagt acaatcttat tcggcacttt attaggtgta acttataaag ctgaagctgc 18121 aacagtacat gtagctggtg gtgtttggag ccatggtatt ggaaaacatt acgtatggtc 18181 ttattatagt cacaataaaa gaaatcatgg ttcaacagca gtagggaaat attcatcttt 18241 tagtggtgtt gctcgacctg gtgttcaatc taaggcatct gctccaaagg cttggggtgg 18301 aaacaaaaca ttttatagtc ttcactaatt acatatatta attaaggaga atagacagaa 18361 ttaagtaaac ttaattctgt ctattctctt gaaagaggca atatgaaaaa attcaaatta 18421 tttttagatt ttagtacttt attattaatt tcagggctat tatttttatt tttcttcaaa 18481 gaaaatgaag aaattatacc tgaaagttcc aatattttaa caatctctaa ttgggataaa 18541 tctaacagta aatcaaaagt gttagatgtc attgaatcag gcgccaaaaa tcaaaatata 18601 caaataatta aatccgttaa agattttgat aataaaaaag aattttttgt tttcaactca 18661 aaacgaaata attctgactt tatacgtaat aaaacatcac ttttaactcc atcagattta 18721 ttaaacagag agataaaagg aaagtattat attattggtg aacatttcaa tgtagaagag 18781 cttacgagtg aattaaaaaa agctggttta acatctgaag ttgaatacat aaatagaaac 18841 atattatttt ttgaattagt catagataat gatttattag tgcctttcat attcttaggc 18901 gtattatatc ttttgtactt cttatatgac agaggatata atttgaaatt ttatgctatt 18961 caaaagctac atggcttttc aactacacgc attatttttc ataatattca tataaaaatc 19021 atttattggc tagtacttac cgctattttc tttattgcta atatattaat cattcatata 19081 gccaatttag aattggattt cttaatgttt attcgtagat ttatcgtgtt gctttttgtt 19141 tttgcttgta ttactacatt gctgtggctg atttcgtata gtcttctttt gaagttaagt 19201 attccattaa tcttaaaagg gaaaaaagga tataagtttt caatatttat gggtaccttc 19261 accaaatgta tcatggtgct aatactatct tttttactgg cacaaaattt aaatgcttat 19321 acgaagttaa agaatatata tgactctgaa aaaatctggc aaaagttaga taattactat 19381 acattagaga tttctcctaa tattagaaat tcagaagaaa aacaaatttt agaaactaat 19441 atatacaatt taattaagaa aagcgaacaa cttggtggat tgcttttaaa gaataataat 19501 atagccaatc ccgataaaaa caattacatt cctgaaaatg gcaatgtctt ttttatcaat 19561 aacaattttc tgaatttcta taaaaatata aatcatgatt ttaaaatgat tgacaatcat 19621 tcggataaaa taaacgtgtt tattcctcca aggttacaat atcaaaaatc tgaaattcaa 19681 aaaaatcacc aaggatggat tgactttcaa aagcaacaaa acaaaaaagt agatgtgcaa 19741 gtactgtcaa aaaacgtatc tgttttttct tttgatcgca caactaattt gaaatttggg 19801 tatctagatt ctccaatagt aatgttgttg acacttgacg atttaacagg agatttttat 19861 ttagctgctg tttctcaagg tggatatcta tttaaagatt tagatacact taaacagttg 19921 cttaaagata atcacctaga agaagaaatt agcggtataa ctaattacaa ggattctgtg 19981 ttaaatgaat taaatgaaac gaaaacaaaa atgataataa caatagttac tattattatt 20041 aacgttttca ttcttttgat agctactgta tttgaaacaa ttcaatattt tagctggaat 20101 aaaaagcaac tattattaag aaaaattcat ggttattcat tattcagcag taatgtacgt 20161 tatttaacaa tttctatctt actttctatt atgttagctt acactacgca tattttattt 20221 ggttcaaaga tattactatt cataataatg tctattgcta ttattcaaca tttacttcaa 20281 attgcttata ttaaatatct tgaaaaatac tttaaagatt taatgaggga gatttaaaca 20341 atgaaaagaa ctttaattat tgtaacaatt gtactattaa tcatcatact tagtctgtta 20401 ttggttagaa atgagcattt agatcgtttt aacccactgt taaaagaaaa agtatcttat 20461 gccaaagttc caaaagatac ccaggactat agaaatataa ctgtatactc taaaaatgga 20521 gaaaaaaaga gttataaatt ggatttttta ggttatgatc ctacaaaaga atatgtgaaa 20581 gtaaatcata aagggaaata tgtgcgttcg atagaataca ttacaaaaat attcctagct 20641 ttttaaaatg ag //