LOCUS       NC_004925               5247 bp    DNA     circular BCT 12-MAR-2008
DEFINITION  Aeromonas salmonicida subsp. salmonicida plasmid pAsa2, complete
            sequence.
ACCESSION   NC_004925
VERSION     NC_004925.1  GI:32453780
KEYWORDS    .
SOURCE      Aeromonas salmonicida subsp. salmonicida A449
  ORGANISM  Aeromonas salmonicida subsp. salmonicida A449
            Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Boyd,J., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M.
  TITLE     Three small, cryptic plasmids from Aeromonas salmonicida subsp.
            salmonicida A449
  JOURNAL   Plasmid 50 (2), 131-144 (2003)
   PUBMED   12932739
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2003) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 5247)
  AUTHORS   Boyd,J.M., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2003) Institute for Marine Biosciences National
            Research Council of Canada, 1411 Oxford St., Halifax, NS B3H 3Z1,
            Canada
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY301064.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Aeromonas salmonicida subsp. salmonicida A449"
                     /mol_type="genomic DNA"
                     /strain="A449"
                     /sub_species="salmonicida"
                     /db_xref="taxon:382245"
                     /plasmid="pAsa2"
     gene            476..1183
                     /locus_tag="ASA_pAsa201"
                     /old_locus_tag="pAsa2_01"
                     /db_xref="GeneID:3722530"
     CDS             476..1183
                     /locus_tag="ASA_pAsa201"
                     /old_locus_tag="pAsa2_01"
                     /note="Orf2"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_861569.1"
                     /db_xref="GI:32453781"
                     /db_xref="GeneID:3722530"
                     /translation="MEGSKEEVVAQLRDDLNSLHEKNKVPVLVREIIEQKRWQGSPQK
                     TAVHYGCPTREEDMGRYNDASGRTGVCYTADFAVTACAESYGRLYHKSDTKFHIGSDD
                     LDKAHICTVRTTRETNTIDMGKLQACLHLTSDKLTGDDKTITREIVNWAANLPCSPYD
                     GVTYRSRHHGGNGTCTAFWNVGGRGDPLETVNMVAVSNYKDRNLECFPQGWEEDDIDG
                     EEILTETLHFEITPNNP"
     gene            complement(1218..1874)
                     /locus_tag="ASA_pAsa202"
                     /old_locus_tag="pAsa2_02"
                     /db_xref="GeneID:3722531"
     CDS             complement(1218..1874)
                     /locus_tag="ASA_pAsa202"
                     /old_locus_tag="pAsa2_02"
                     /note="Orf3"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_861570.1"
                     /db_xref="GI:32453782"
                     /db_xref="GeneID:3722531"
                     /translation="MNRWKDSVMKTQTVQHAAPMAKERATLSHILSKRVEGLIGSVLP
                     EIEQGAPIHTALELTDNDLMKLIVASFISKNSRVSPREMNKIKRRIEGTFSFYDLLKS
                     LGGTIRVNEVASLLNVTRQTVHNYIKRNELLAVKPGNEFLFPIFQFDNDGISSEFQKI
                     LKLLPDDISPVTKISFFTSTVVEKENKSIIDILKTNNITSNTLSIIRNRARVFGRQMG
                     "
     gene            1998..2537
                     /locus_tag="ASA_pAsa2s1"
                     /old_locus_tag="pAsa2_s1"
                     /db_xref="GeneID:3722529"
     misc_RNA        1998..2537
                     /locus_tag="ASA_pAsa2s1"
                     /old_locus_tag="pAsa2_s1"
                     /product="misc_RNA"
                     /note="replication primer; oriV region; RNAII"
                     /db_xref="GeneID:3722529"
     gene            complement(1998..2110)
                     /locus_tag="ASA_pAsa2s2"
                     /old_locus_tag="pAsa2_s2"
                     /db_xref="GeneID:3722528"
     misc_RNA        complement(1998..2110)
                     /locus_tag="ASA_pAsa2s2"
                     /old_locus_tag="pAsa2_s2"
                     /product="misc_RNA"
                     /note="antisense RNA; regulator of RNAII"
                     /db_xref="GeneID:3722528"
     rep_origin      2518
                     /locus_tag="ASA_pAsa2s1"
                     /old_locus_tag="pAsa2_s1"
                     /note="DNA replication origin"
     oriT            2790..2808
                     /note="origin of transfer"
     gene            2977..3282
                     /locus_tag="ASA_pAsa203"
                     /old_locus_tag="pAsa2_03"
                     /db_xref="GeneID:3722532"
     CDS             2977..3282
                     /locus_tag="ASA_pAsa203"
                     /old_locus_tag="pAsa2_03"
                     /note="Orf1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_861571.1"
                     /db_xref="GI:32453783"
                     /db_xref="GeneID:3722532"
                     /translation="MKDERDDQTLDLLPSGKRRGLPPTGKALSAAAKQAAYRARQREK
                     TVTVTLNRLDCGELEIFLRNLRDGRTSTLAPEVVARLHEAVRSAWRGQLHSGNGDQK"
     gene            3303..3620
                     /gene="mobC"
                     /locus_tag="ASA_pAsa204"
                     /old_locus_tag="pAsa2_04"
                     /db_xref="GeneID:1496850"
     CDS             3303..3620
                     /gene="mobC"
                     /locus_tag="ASA_pAsa204"
                     /old_locus_tag="pAsa2_04"
                     /note="MobC"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861572.1"
                     /db_xref="GI:32453784"
                     /db_xref="GeneID:1496850"
                     /translation="MIKIRVTADELVRLKGLSGDERLAEWMRSKCLGNDKAAGRRRTP
                     VPKADPALLRQIAGIGNNLNQVARRVNSGEWGAVERVQVIAVLMAMERALQAISVPST
                     EVT"
     gene            3787..5247
                     /gene="mobA"
                     /locus_tag="ASA_pAsa205"
                     /old_locus_tag="pAsa2_05"
                     /db_xref="GeneID:1496851"
     CDS             3787..5247
                     /gene="mobA"
                     /locus_tag="ASA_pAsa205"
                     /old_locus_tag="pAsa2_05"
                     /note="MobA"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization relaxase"
                     /protein_id="NP_861573.1"
                     /db_xref="GI:32453785"
                     /db_xref="GeneID:1496851"
                     /translation="MIVKFHARGVGRGSGPVDYLLGPQRDREGATLDRGDPGLIEALI
                     DSSPYAKKYTSGVLSFQEADLPREDKDKLMDSFERALLPGLDRDQYACLWVEHRDKER
                     LELNFVIPNIELLSGKRLQPYFDRADRPRIDAWKVVVNAQLGLHDPDDPLNKRALMTP
                     SNLPKAKQDAAQAITDGLLSLAAAGELADRGDVVATLKQHGFEVVRETRKSISIADPE
                     GGRNIRLKGALYEQDFRAGQDLRKELEAAGERYRNEREERVRAARESYSRGVGLKRAE
                     IERRYRRPEPEVGLAGVEGVGFSVDRGGISAGDQLGRGVVPGEGHRRELGGDLAPEQD
                     IGAVDGEGRQIGTVNVRRGQAAVREGGLEGVGLWRQRERRISLDDPGGLLNDRARTAV
                     IERVREFGRRVQSAALGLGERLQRFAADVRGHLYREPGIAGERGELEQAGVALERSSR
                     EIEPKIEALSLDREPRCGGMSFGREYREYGDREYGD"
     gene            4475..4939
                     /gene="mobB"
                     /locus_tag="ASA_pAsa206"
                     /old_locus_tag="pAsa2_06"
                     /db_xref="GeneID:1496852"
     CDS             4475..4939
                     /gene="mobB"
                     /locus_tag="ASA_pAsa206"
                     /old_locus_tag="pAsa2_06"
                     /note="MobB"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861574.1"
                     /db_xref="GI:32453786"
                     /db_xref="GeneID:1496852"
                     /translation="MSKILELAKTFEKSSKQQASDIETSVKNAFEPHEKAILEALDSS
                     GRRLNAAIDAQSRRWGWLVLKGWVFPLIGVAFLLGISWVVVWYQGRVIAENWVEISRQ
                     NKTLEQLTAKGGKLELSTCGEDKRLCVKVDLKELAYGDKEKDEYPWMIPEGY"
     gene            4940..5164
                     /gene="mobD"
                     /locus_tag="ASA_pAsa207"
                     /old_locus_tag="pAsa2_07"
                     /db_xref="GeneID:1496853"
     CDS             4940..5164
                     /gene="mobD"
                     /locus_tag="ASA_pAsa207"
                     /old_locus_tag="pAsa2_07"
                     /note="MobD; putative"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861575.1"
                     /db_xref="GI:32453787"
                     /db_xref="GeneID:1496853"
                     /translation="MTELERQLLNALESLEGGYRVQHSAWESAYSDLRLMFEATSTEN
                     RALRESVGSLSKQVLHLSAQVERLSQKLKP"
ORIGIN      
        1 gtggccctac tcaatcatta gccggtatag ctgttgcgac gtacattttc gttccgttgg
       61 acttcatacc ccataacccg taggaataca cttcaaacgc gcttaggcgc gttttttgtt
      121 ttcggtatgc cattgccttg ggggtaggga agaaatggct gtaatcgcca caagtaaagc
      181 ctgtttttgg agctatttca tagctcgtag ctaaattgtg tttgtttgat taaaaaacta
      241 gcaaaccacc ataaattcaa tctaggcgtt tttgtacgat tttggttttc ttatccttat
      301 tttcaataag gaaagtttat aggcgttgat tgttgggata caatgtctct gccatgaaaa
      361 atatggcaag gaggatgctg gctaaagcta atgcatcttt aaataaacat aagcatacaa
      421 aggtgaagct tatgcaatta attataaacc cacactcacg atatcgaggt atcaaatgga
      481 aggaagtaag gaagaagttg ttgcacaact acgtgatgat ttgaattctc tacatgagaa
      541 aaataaagtt cccgtacttg ttcgtgaaat catagagcaa aagcgttggc agggaagtcc
      601 gcaaaaaacg gcagtccatt atgggtgccc tacgcgtgag gaagatatgg ggcgttataa
      661 tgatgcttct ggtcgaactg gcgtttgtta taccgcggat tttgcagtaa ctgcgtgcgc
      721 tgagtcatat ggtcgccttt accataagtc agataccaag tttcacattg gtagtgatga
      781 tctggataag gctcacattt gcacggtcag gactacgcgt gaaacgaaca ccattgatat
      841 gggaaaactg caggcttgcc tgcatttgac tagtgataaa ttgactggtg atgacaaaac
      901 catcaccagg gagattgtca actgggctgc gaatcttccc tgttcgcctt acgacggagt
      961 tacataccgt tcaaggcatc atggtggtaa cgggacgtgc acagccttct ggaatgttgg
     1021 tggaagggga gaccctttgg aaactgtcaa tatggttgct gtaagcaact ataaagatcg
     1081 gaatctagag tgttttcctc aagggtggga agaggatgat atcgatggtg aggaaatctt
     1141 aacagaaacc cttcacttcg aaatcacccc taacaaccca taataaagat ggtgtcgcca
     1201 gttaatggcg acacttctta ccccatctgg cgcccaaaaa ctcgcgctct atttcttata
     1261 atacttaaag tattgctagt tatattattt gtttttaaaa tgtcaattat tgatttgttt
     1321 tctttttcta caacagtgga tgtgaaaaat gaaatcttag tcactgggct gatatcatca
     1381 ggaagtaact taagtatttt ctggaactca cttgagatcc catcgttgtc aaattgaaat
     1441 attgggaata aaaactcatt acctggttta acggctaaca gttcatttct ttttatatag
     1501 ttgtgcacag tttgtctcgt aacatttaat agcgatgcta cttcattcac cctgattgtg
     1561 ccacctaagg attttagcaa atcataaaaa gagaaagtcc cttctattct tcgctttatt
     1621 ttgttcattt cccgagggct gactcgtgaa tttttagata tgaaacttgc tacgatcagt
     1681 ttcatcagat cattatcggt taactctagg gccgtatgaa taggggctcc ctgttcaatt
     1741 tcaggaagaa cagagcctat taggccttcg actcttttgc taagaatatg gcttagggtt
     1801 gcacgctctt ttgccattgg ggcagcatgt tgcacagttt gagttttcat cacactatcc
     1861 ttccacctat tcatttacgt ttatgttacg ccggtatgtg taaaacgtca atcttggtgc
     1921 aaggatgcat tattgggttt ggttacggtt caaatgatct tgatcagatc gtctgatcaa
     1981 gcgtaacctt ctaagcagaa aacaaaaaac ccgcctttag gcgggttctt tgcccggtgt
     2041 tctaaggcgt aggaaccttg gaatccggtg ggtaacagct tcgagcagga agcggatacc
     2101 gttaagtacc cccccagttt agccgcgtgc ggcccactgt tcaagtatca aacccctgct
     2161 cgaaccctgt taccaaaggc tttcaccttt ggcgctttgt cgtattcgct cggattggac
     2221 gcaagtgatt gttgccgtag cgggtgtgaa ggtcgggcag aacggggaaa tctcacatac
     2281 tgcccaagcg tgcaatcgcg caccacagcc tcccagggct cgcaaccctg gggcatctgc
     2341 aagtagagcg taacggggca ccaaccacgg ccagctctta aaaaacggcg cggcagcccg
     2401 accaacaggc gggggctaaa gcagagtgca gcgggacggg gtgtgttctc tggagcgcgt
     2461 gagagggagc tgccgcaagc agtctcgata gtccggagca aggcgagctt atcctgtaaa
     2521 agaccctaaa tcgatgtggg tctgtaggag ggctctccta tcttgaagcg cagcgaaaga
     2581 taggagagcc cggcaaactg cggcggcttg ccaacatcct tttcgtccgt ttttttgctt
     2641 ccttagggca cgcatcatca cctcccttga tctctttttg cttcctacct gctacctatc
     2701 ctcgaagccc tccaattaca gtcactgtta ctctaaatag tctcactaac aggtggttac
     2761 aacctattcc gcctatgtca aacttgactc taagccagca tacacagagt gcccttagcc
     2821 gcttcgcgtc ttcggttcct ctgtgaagct ggttcagggg cgtgccgccc ccggaccccc
     2881 ctgcctccac cgccccgaaa aaccccgttc cgggctttcc caggtcggcg gacagaagag
     2941 gtctatggtc acagttaccg taattgaggt gatcggatga aggacgaacg agacgaccag
     3001 accctggact tgctgccaag tggcaagcgc cgaggtctgc cacctaccgg caaggcgttg
     3061 agcgccgccg ccaagcaagc tgcttaccgg gcacggcaac gggaaaaaac ggtcaccgtt
     3121 accttaaatc gtctggattg tggcgagctg gagattttcc tgcggaacct acgggatggc
     3181 cgcacctcga ctctggcccc tgaggtggtg gccaggttgc atgaggctgt gcggtcggca
     3241 tggcgggggc agcttcattc cggtaacggt gaccaaaaat gaccccgacc aaacgcgaac
     3301 gggtgatcaa gatccgggtc accgctgacg aactggtccg cctaaagggc ttgagtggtg
     3361 atgaacggtt ggccgaatgg atgcggagca agtgccttgg gaacgacaaa gcagccggtc
     3421 ggcggcgaac gccggtaccc aaagccgatc cggctctgct gaggcagata gccgggatcg
     3481 gcaacaacct caaccaggta gcgcggcgag tgaatagcgg ggagtggggc gcggttgagc
     3541 gtgttcaggt gatcgcggtg ctgatggcga tggagcgtgc tttgcaggcc atctctgtac
     3601 cctcaacgga ggtaacgtga tgaacgaata cagcgtagcc gagtatgact acaccctgat
     3661 ccggttgccc tgcgaacagg gggggagctt gcggttgttg aaggatggcc aggaggtcag
     3721 tggggaggtt taccaggagc acgatgaggc gctcagtgtg gccaccgtct ggctgtgcag
     3781 cgagtcatga tcgtcaaatt tcatgcccgt ggcgtagggc gcggtagcgg cccggtcgat
     3841 tacctgctgg ggccgcagcg ggatcgggag ggggcgaccc ttgaccgcgg cgatccgggt
     3901 ctcatcgagg cgttgatcga cagtagcccc tacgccaaaa agtacacatc cggcgtgctg
     3961 tcgttccagg aagcggactt gccccgggag gacaaggaca agctgatgga cagcttcgag
     4021 cgtgcgttgt tgccagggtt ggatcgggat cagtacgcct gcctgtgggt ggagcaccgg
     4081 gacaaagagc ggcttgagct caacttcgtg atcccgaata tcgagctgct gagtggcaag
     4141 cggctgcaac cctatttcga ccgggccgat cggccccgca tcgatgcgtg gaaggtggtg
     4201 gtcaacgccc agttgggatt gcatgacccg gatgatccct tgaacaaacg ggcattgatg
     4261 acccccagca atctgccaaa ggccaagcag gacgcggcac aagcgatcac cgacggtctg
     4321 ctttcgctgg cggcggccgg tgagcttgcc gaccggggag acgtggtggc cacgttgaag
     4381 caacacggct ttgaggtggt gcgggagacc cgcaagagta tcagcattgc cgacccggaa
     4441 ggggggcgga atatccggct aaaaggggcc ctgtatgagc aagattttag agctggccaa
     4501 gaccttcgaa aagagctcga agcagcaggc gagcgatatc gaaacgagcg tgaagaacgc
     4561 gttcgagccg cacgagaaag ctattctcga ggcgttggac tcaagcgggc ggagattgaa
     4621 cgccgctatc gacgcccaga gccggaggtg gggctggctg gtgttgaagg ggtgggtttt
     4681 tccgttgatc ggggtggcat ttctgctggg gatcagctgg gtcgtggtgt ggtaccaggg
     4741 gagggtcatc gccgagaact gggtggagat ctcgcgccag aacaagacat tggagcagtt
     4801 gacggcgaag ggcggcaaat tggaactgtc aacgtgcggc gaggacaagc ggctgtgcgt
     4861 gaaggtggac ttgaaggagt tggcctatgg cgacaaagag aaagacgaat atccctggat
     4921 gatcccggag ggctactaaa tgacagagct cgaacggcag ttattgaacg cgttagagag
     4981 tttggaaggc gggtacagag tgcagcactc ggcctgggag agcgcctaca gcgatttgcg
     5041 gctgatgttc gaggccacct ctaccgagaa ccgggcattg cgggagagcg tggggagctt
     5101 gagcaagcag gtgttgcact tgagcgctca agtcgagaga ttgagccaaa aattgaagcc
     5161 ctgagcctgg atcgggagcc gcggtgtggt ggaatgagct tcgggcggga gtatcgagag
     5221 tatggtgatc gggagtacgg tgattga
//
