LOCUS       NC_004924               5616 bp    DNA     circular BCT 12-MAR-2008
DEFINITION  Aeromonas salmonicida subsp. salmonicida plasmid pAsa3, complete
            sequence.
ACCESSION   NC_004924
VERSION     NC_004924.1  GI:32453769
KEYWORDS    .
SOURCE      Aeromonas salmonicida subsp. salmonicida A449
  ORGANISM  Aeromonas salmonicida subsp. salmonicida A449
            Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 5616)
  AUTHORS   Boyd,J., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M.
  TITLE     Three small, cryptic plasmids from Aeromonas salmonicida subsp.
            salmonicida A449
  JOURNAL   Plasmid 50 (2), 131-144 (2003)
   PUBMED   12932739
REFERENCE   2  (bases 1 to 5616)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2003) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 5616)
  AUTHORS   Boyd,J.M., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2003) Institute for Marine Biosciences National
            Research Council of Canada, 1411 Oxford St., Halifax, NS B3H 3Z1,
            Canada
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY301065.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5616
                     /organism="Aeromonas salmonicida subsp. salmonicida A449"
                     /mol_type="genomic DNA"
                     /strain="A449"
                     /sub_species="salmonicida"
                     /db_xref="taxon:382245"
                     /plasmid="pAsa3"
     gene            123..347
                     /gene="relB"
                     /locus_tag="ASA_pAsa301"
                     /old_locus_tag="pAsa3_01"
                     /db_xref="GeneID:1496843"
     CDS             123..347
                     /gene="relB"
                     /locus_tag="ASA_pAsa301"
                     /old_locus_tag="pAsa3_01"
                     /note="RelB"
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="NP_861560.1"
                     /db_xref="GI:32453770"
                     /db_xref="GeneID:1496843"
                     /translation="MLAIRLPSEIEERLANLAQETGRTKTFYAREAILTHLEELEDYY
                     LAERVMENVRKGEERLFSLDDVERDLGLES"
     gene            313..600
                     /gene="relE"
                     /locus_tag="ASA_pAsa302"
                     /old_locus_tag="pAsa3_02"
                     /db_xref="GeneID:1496844"
     CDS             313..600
                     /gene="relE"
                     /locus_tag="ASA_pAsa302"
                     /old_locus_tag="pAsa3_02"
                     /note="RelE"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="NP_861561.1"
                     /db_xref="GI:32453771"
                     /db_xref="GeneID:1496844"
                     /translation="MTWSAILAWKVELTDTAKKQLARLDKTQSQRITKYLRRIMMLEN
                     PRDAGKALTGNLRTYWRYRVGDYRVVCDIRDNDLVIVAVIIGHRSEVYGGS"
     gene            709..1338
                     /locus_tag="ASA_pAsa303"
                     /old_locus_tag="pAsa3_03"
                     /db_xref="GeneID:3722527"
     CDS             709..1338
                     /locus_tag="ASA_pAsa303"
                     /old_locus_tag="pAsa3_03"
                     /note="Orf4"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_861562.1"
                     /db_xref="GI:32453778"
                     /db_xref="GeneID:3722527"
                     /translation="MKAIIPLLLALIPLIYIYLKHYAKKANGIVQANLALERLDAQLT
                     SESWWGAKNEFNTLTLNSLKYPEMILYMEKLPVTKRNTRCVSYMKLISVLDELERIPN
                     DVLLSNQDKFDVSSMEMDCLFNKLWKDFDIKAINEDIIAAKIDIVLSIAQSILYFARN
                     GETTQAKRAIMRAGGDEPPLETMHLREEAASVVEFFLLELSSIKKLTAN"
     gene            complement(1772..1840)
                     /locus_tag="ASA_pAsa3s01"
                     /old_locus_tag="pAsa3_s01"
                     /db_xref="GeneID:3722525"
     misc_RNA        complement(1772..1840)
                     /locus_tag="ASA_pAsa3s01"
                     /old_locus_tag="pAsa3_s01"
                     /product="misc_RNA"
                     /note="antisense RNA; regulator of rep; RNAI"
                     /db_xref="GeneID:3722525"
     gene            1914..2900
                     /gene="rep"
                     /locus_tag="ASA_pAsa304"
                     /old_locus_tag="pAsa3_04"
                     /db_xref="GeneID:1496845"
     CDS             1914..2900
                     /gene="rep"
                     /locus_tag="ASA_pAsa304"
                     /old_locus_tag="pAsa3_04"
                     /note="Rep; ColE2-type replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="replication primase"
                     /protein_id="NP_861563.1"
                     /db_xref="GI:32453772"
                     /db_xref="GeneID:1496845"
                     /translation="MVKPALQAAAFVERLPRRPYCTDDPAHGLLIRPQATALAYRHIQ
                     HNPPPHVACLVFDVDSPDGYEAWKEAGLPAPNWVTVNPLNCHAHYGYYLAAVVARTSA
                     AKQKPLRYLAAIEHVLAKRLGADMGYAGLITKNPVHGDWWTIWHHSEPFSLDYLAEFC
                     PDADLAAYSRRSRKEVGGLGRNVTVFDNVREWAYSAVRAYWRPNGYEAWGDAVRSACD
                     SANAFGREQGGPLPVSEIKATAKSIARWVWNRFTPAGFSLVQAHRGAKGGRIGGKLSK
                     GGGRPSNSGKVRNDLLPEVIRLKAMGYSNRDIPEDLQISPGSVSNYLRRDRE"
     rep_origin      2912..2930
                     /note="oriV"
     gene            complement(3015..3338)
                     /locus_tag="ASA_pAsa305"
                     /old_locus_tag="pAsa3_05"
                     /db_xref="GeneID:3722526"
     CDS             complement(3015..3338)
                     /locus_tag="ASA_pAsa305"
                     /old_locus_tag="pAsa3_05"
                     /note="Orf1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_861564.1"
                     /db_xref="GI:32453773"
                     /db_xref="GeneID:3722526"
                     /translation="MKDSTDKGTGDMFGEKRGRGRPKTGTAKTGAERQAAFRAKQQEN
                     NVTVTISRELLDGLDAQMQAIRDRGSVVVLTPEQAGEILKAIRKSSLKQLRGQRPRPQ
                     AKPTA"
     oriT            3437..3455
                     /note="origin of transfer"
     gene            3549..3959
                     /gene="mobC"
                     /locus_tag="ASA_pAsa306"
                     /old_locus_tag="pAsa3_06"
                     /db_xref="GeneID:1496846"
     CDS             3549..3959
                     /gene="mobC"
                     /locus_tag="ASA_pAsa306"
                     /old_locus_tag="pAsa3_06"
                     /note="MobC"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861565.1"
                     /db_xref="GI:32453774"
                     /db_xref="GeneID:1496846"
                     /translation="MTKTARTTTVSVRIDKPTAAEWREKAEAADLSLSDWIRGAIDAN
                     QQMHLPTPRKRAVRVRDTSHDADPVLMRQLAGMSNNLNQIARALNECRLIGSPVQVVE
                     VLALLRSIEGQATHLLPQLPPPPKPNRKGESDAH"
     gene            3949..5616
                     /gene="mobA"
                     /locus_tag="ASA_pAsa307"
                     /old_locus_tag="pAsa3_07"
                     /db_xref="GeneID:1496847"
     CDS             3949..5616
                     /gene="mobA"
                     /locus_tag="ASA_pAsa307"
                     /old_locus_tag="pAsa3_07"
                     /note="MobA"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization relaxase"
                     /protein_id="NP_861566.1"
                     /db_xref="GI:32453775"
                     /db_xref="GeneID:1496847"
                     /translation="MHIKFLAHGTGSGAHASAYLMGPHDHTGEPRAGVAVLRGDPVLF
                     AAVADSLPFQQRYSSAVISWAAEEAPRPDEISAVLDDFEALAFAGLAREDVHLTAVEH
                     AEADGGVHVHILIPRVHLGSGKSFNPAPPGQRKDFDAVRDKHNHEKGWARPDDPLRAR
                     LLRPDFEAYKAVTDHTAIKKQLTDHLIGAVAQGVVSNADELRAYLQEGLGCEITRTGK
                     DYTSVKPEGYPKAIRLKGELYGESWTAQSTLEREAQAAARAGIGRGGEVSASAARDAQ
                     ERLAGAHQRRAEYNRDRYQGRDRGLAPADLRREQTLENSLEGGQGRSRHAETGLQRLV
                     QSRAAGDFLRGGVTDGKLVWHELDMGADRTRHGDVAEFAARDQQRPEHVGRVAEGGEL
                     LQGGGWKTLHHRPQDWGAVQDRGGQDGRGGALNDRAGAGTVGEAGADRTAARATNRGL
                     EAAIAAASALFERVAKGVQRCAQELREAMQRPARGDPDVAERGNALEQGYQRSARELE
                     QQCQRLEQGAGELAERAGLINEQAKELKESKELELSLEYERYRGLEL"
     gene            4664..5221
                     /gene="mobB"
                     /locus_tag="ASA_pAsa308"
                     /old_locus_tag="pAsa3_08"
                     /db_xref="GeneID:1496848"
     CDS             4664..5221
                     /gene="mobB"
                     /locus_tag="ASA_pAsa308"
                     /old_locus_tag="pAsa3_08"
                     /note="MobB"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861567.1"
                     /db_xref="GI:32453776"
                     /db_xref="GeneID:1496848"
                     /translation="MESLGQRKALLSEKLKQRQEQELGEVEKLAQALLETLRSDLQER
                     ISGELNTTVTAIKEGIEDLPQQIYDASRPLKTVLREVKDEADMLKLVSSVWFKAALLG
                     IFCGVGLLMGSWFGMNWIWEQIEQDMGTLRSLRQEISSAQSTLDELPKGVSYFKGEDG
                     KRYIIARKIGAPYKTEAGKMAVEVR"
     gene            5222..5530
                     /gene="mobD"
                     /locus_tag="ASA_pAsa309"
                     /old_locus_tag="pAsa3_09"
                     /db_xref="GeneID:1496849"
     CDS             5222..5530
                     /gene="mobD"
                     /locus_tag="ASA_pAsa309"
                     /old_locus_tag="pAsa3_09"
                     /note="MobD; putative"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861568.1"
                     /db_xref="GI:32453777"
                     /db_xref="GeneID:1496849"
                     /translation="MTELERVLLAKLEQIEQRHEQQTEDLRLQLQQQAHSLSALQKVC
                     NDALRSCGKLCSDLHEEIRTLQSGVTHSNKVTSAALGSLNSSVSALNKALENLQSAQG
                     "
ORIGIN      
        1 ctgccgcgcg ttgcttggcg gcccctgcaa cgagattgac agacttcgct agtcacccta
       61 gttgcagggg ctggagttta gcgtatgata tacacgtata tcattagcct ttggggtgga
      121 ttatgttagc gatacgcttg ccttcggaga tcgaagagcg tttggcaaat ttggcgcaag
      181 agacgggaag aacgaagacc ttctatgcac gggaggccat cctgacccat ctggaagagt
      241 tggaagatta ctatctggct gagagggtca tggagaatgt tcgcaagggc gaagagcgcc
      301 tgttctcatt ggatgacgtg gagcgcgatc ttggcttgga aagttgaatt gaccgatacc
      361 gccaagaagc aattggctag gctggataag acccaatcgc agaggatcac aaaatatctg
      421 cggcggatta tgatgcttga aaatccgagg gatgctggca aagctctaac cggtaattta
      481 cgaacctact ggcgttatcg cgtgggggat tatcgggttg tgtgtgacat aagagataac
      541 gatcttgtca ttgttgctgt gataattggg catcgcagtg aagtatatgg tggaagttag
      601 tttttgttac tgactgtctt ggtaggaact acaacagatg attgataatg aattacagaa
      661 atcatctgct cacaccaaat gcattaaatt aacaagtgag atttattaat gaaagccata
      721 attccactgc tacttgctct tatacctctt atatatattt atctaaagca ttacgcaaaa
      781 aaagccaatg gaatagtcca ggcaaatttg gcgctagagc gtcttgatgc tcagttaaca
      841 tcagagagtt ggtggggggc gaaaaatgaa ttcaacaccc tgactttaaa ttcattaaaa
      901 tatccagaaa tgattttata tatggaaaaa ttgcctgtaa caaaaagaaa tactaggtgc
      961 gtgtcatata tgaaactaat tagtgtgttg gatgaattgg aaagaattcc taatgatgtt
     1021 cttctgagca accaagacaa gtttgatgtt agcagcatgg aaatggattg tcttttcaat
     1081 aagctgtgga aagatttcga tataaaagct atcaatgaag acattatagc tgctaagatc
     1141 gatattgttt tatccatagc gcaatcaatt ctttattttg caagaaatgg tgaaaccacc
     1201 caagcaaaaa gagctataat gagagctggc ggagatgagc caccactaga aacaatgcat
     1261 ttgcgcgaag aggcagcttc tgttgttgaa ttcttccttc ttgaactctc ttctataaaa
     1321 aagctaaccg cgaattaaga taataagtgc aacgcgtgag gaggttgtgg tgagtcggct
     1381 aactgccggg tcactccaca cattgtccgc gaaagacgag cgaaaagatg tacgagcagc
     1441 taactgaagg ccaacgaggg ggggggctag gggttataag ccggaagcca agaaaattcc
     1501 gctggtaaac gtttaggctg atctttccgt ctacaaattg tatccgacag ggtttgccgt
     1561 atatgttttg ctcatagctg cgcgtatttt tcgacccaat acgggctatg tcgggaagca
     1621 actgttttac gacaactcgc gccaacactg gtttttctgg cgagacggaa aaaactggaa
     1681 ttccggcgta gaacctcaga tcggacatcg tagtgaggtg tatggctaaa cgttgctcag
     1741 gcgtaggcac ctgcggtaac gtactgtcca gaaaagacaa accccgaatc tctgttttca
     1801 acttggcgga agagcacaga gagttcgggg ccggacttat gcaaagcaat aggtgaaata
     1861 tatcacatgg gtcacaccaa acaagccagc accgccaata agcgcggtgt ccaatggtaa
     1921 aaccagcgct tcaagccgct gcctttgtcg aacgtctccc ccgccgcccc tactgcaccg
     1981 atgatccggc gcatgggctg ctgatccgcc cgcaagcgac cgcgctggct tatcgccata
     2041 tccagcacaa cccgccgccg cacgtggcct gtctggtgtt cgatgtggac agcccagacg
     2101 gctatgaggc gtggaaggag gccggattac ccgccccgaa ctgggtcacg gtcaaccctc
     2161 tcaattgtca cgctcactac ggatactatc tagcggctgt cgtggcccgt accagtgcgg
     2221 ccaagcaaaa gccgttgcgc tatctggcgg ccatagaaca cgttctggcg aagcggttgg
     2281 gggccgatat gggatatgca gggcttatca ccaaaaaccc cgtacatggc gattggtgga
     2341 cgatatggca tcacagcgag ccgtttagcc tcgactatct ggcggagttc tgccctgatg
     2401 cggatttggc ggcctatagt cgccgcagtc gcaaagaggt cggcggactg ggccgtaacg
     2461 tcacggtgtt tgataacgtg cgtgaatggg cctacagcgc cgttagggcg tattggcggc
     2521 ctaacgggta cgaggcttgg ggcgatgcgg tgagatctgc ttgtgatagc gctaatgcgt
     2581 ttggacggga gcagggcggc ccgcttccag tgtcagagat caaggccacg gcaaagagta
     2641 tcgcccgttg ggtatggaat cgattcaccc ccgccgggtt tagcctggtg caggcgcatc
     2701 gaggagcgaa gggtggacgc atagggggga agctatccaa aggcggtggc aggccgagca
     2761 atagtgggaa agttcgcaat gacttgctcc ccgaagtgat caggctcaaa gctatgggtt
     2821 acagtaaccg cgatattcca gaggatctgc aaatttcgcc gggcagcgtg tccaattacc
     2881 tccgtcgtga tcgtgagtga tcaaaacaaa gccttatcag ataacagcgc cccgtaaggg
     2941 gcgttttcgc ttctcaaagt attcgagaga gaaccttggc gaggtgggca agcgcccgaa
     3001 ccgtaggccg tccatcaggc cgtgggtttg gcctgtgggc gggggcgttg tccacggagc
     3061 tgctttaggc ttgatttgcg tatggccttc aagatctcgc ccgcctgttc cggtgtcagc
     3121 accacgaccg aaccgcgatc cctgatcgcc tgcatttggg catccagtcc atccagcagt
     3181 tcccgactta tcgttactgt aacgttattc tcctgctgtt tggcacgaaa ggctgcttga
     3241 cgttccgccc cagtctttgc ggttcctgtc ttgggtctac cccgtccgcg cttctcgcca
     3301 aacatatctc ccgttccctt gtcggtgctg tccttcatag ccactccatt tttagtcact
     3361 gtgacaataa aagattatgc gttattgtaa cggtaattac caatttttca ggccccagct
     3421 tgcatgctaa cttggattaa gccagcctac ccgtattact gtttcgcccg cttcgcggct
     3481 cacagtaaca cggggctggt gaggggcttc gccccttcaa tccccacaca aaaaacaccg
     3541 gaggcaccgt gaccaaaacg gccagaacga ccaccgtcag cgtgcggata gacaagccga
     3601 ccgccgccga atggcgagag aaggcggagg cggcagacct cagtttgtcg gactggatca
     3661 ggggggctat cgatgccaat caacaaatgc acctgccgac cccgcgcaag cgagcggtcc
     3721 gagtgcgaga taccagccac gatgcggatc cggtgctgat gcggcaattg gcagggatga
     3781 gcaacaacct caaccagatc gcccgcgccc tgaacgagtg ccggctgatc ggttcaccgg
     3841 tgcaagtggt cgaagtgctg gccctgcttc gcagtatcga ggggcaggcc acgcacctgc
     3901 tgccccagtt gccgccgccc cccaagccga accgcaaggg ggagagcgat gcacattaag
     3961 ttccttgccc acggcacggg aagcggtgct cacgcctcgg cctacctcat ggggccgcac
     4021 gatcacacag gggagccacg ggctggggtg gcggtactgc ggggggatcc ggtgctgttc
     4081 gcggcggtgg ccgactcgct gccctttcag cagcgctata gcagcgcggt gatctcatgg
     4141 gctgccgaag aggccccgcg accggacgag atcagcgctg tgctggacga cttcgaggcg
     4201 ctggcatttg ccggactggc gcgggaggac gtgcatttga ccgcggttga gcacgccgaa
     4261 gcggatggcg gggtgcatgt gcatatcctg atcccccgtg ttcacctggg cagcggcaaa
     4321 tcgttcaatc cggccccgcc agggcagcgc aaagacttcg atgcggtgag ggacaagcac
     4381 aaccacgaaa aggggtgggc gcggccagac gacccgctca gggcgcggct gttgcggccc
     4441 gattttgaag cgtacaaggc cgtgacagac cacacagcca tcaaaaagca gctcaccgac
     4501 cacctgatcg gtgcagtcgc gcaaggggtg gtgagcaatg ctgatgaact gcgggcctac
     4561 ctgcaagagg ggctagggtg cgagataacg cgcacaggca aggattacac cagcgtcaag
     4621 ccggagggat acccgaaggc gatccggttg aaaggggagt tgtatggaga gtcttggaca
     4681 gcgcaaagca ctcttgagcg agaagctcaa gcagcggcaa gagcaggaat tgggcgaggt
     4741 ggagaagtta gcgcaagcgc tgctagagac gctcaggagc gacttgcagg agcgcatcag
     4801 cggcgagctg aatacaaccg tgaccgctat caaggaaggg atcgaggact tgccccagca
     4861 gatctacgac gcgagcagac ccttgaaaac agtcttgagg gaggtcaagg acgaagccga
     4921 catgctgaaa ctggtctcca gcgtctggtt caaagccgcg ctgctgggga ttttctgcgg
     4981 ggtggggtta ctgatgggaa gctggtttgg catgaactgg atatgggagc agatcgaaca
     5041 agacatgggg acgttgcgga gtttgcggca agagatcagc agcgcccaga gcacgttgga
     5101 cgagttgccg aagggggtga gctacttcaa gggggaggat ggaaaacgct acatcatcgc
     5161 ccgcaagatt ggggcgccgt acaagacaga ggcgggcaag atggccgtgg aggtgcgcta
     5221 aatgacagag ctggagcggg tactgttggc gaagctggag cagatcgaac agcggcacga
     5281 gcaacaaacc gaggacttga ggctgcaatt gcagcagcaa gcgcactctt tgagcgcgtt
     5341 gcaaaaggtg tgcaacgatg cgctcaggag ctgcgggaag ctatgcagcg acctgcacga
     5401 ggagatccgg acgttgcaga gcggggtaac gcactcgaac aaggttacca gcgcagcgct
     5461 agggagcttg aacagcagtg tcagcgcctt gaacaaggcg ctggagaact tgcagagcgc
     5521 gcagggctga tcaacgagca ggccaaggag ctcaaggaga gcaaagagct ggagttatcg
     5581 ctggagtatg agcggtatag agggcttgag ctgtag
//
