LOCUS       NC_004923               5424 bp    DNA     circular BCT 12-MAR-2008
DEFINITION  Aeromonas salmonicida subsp. salmonicida plasmid pAsa1, complete
            sequence.
ACCESSION   NC_004923
VERSION     NC_004923.1  GI:32453760
KEYWORDS    .
SOURCE      Aeromonas salmonicida subsp. salmonicida A449
  ORGANISM  Aeromonas salmonicida subsp. salmonicida A449
            Bacteria; Proteobacteria; Gammaproteobacteria; Aeromonadales;
            Aeromonadaceae; Aeromonas.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Boyd,J., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M.
  TITLE     Three small, cryptic plasmids from Aeromonas salmonicida subsp.
            salmonicida A449
  JOURNAL   Plasmid 50 (2), 131-144 (2003)
   PUBMED   12932739
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUL-2003) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 5424)
  AUTHORS   Boyd,J.M., Williams,J., Curtis,B., Kozera,C., Singh,R. and Reith,M.
  TITLE     Direct Submission
  JOURNAL   Submitted (16-MAY-2003) Institute for Marine Biosciences National
            Research Council of Canada, 1411 Oxford St., Halifax, NS B3H 3Z1,
            Canada
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AY301063.
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Aeromonas salmonicida subsp. salmonicida A449"
                     /mol_type="genomic DNA"
                     /strain="A449"
                     /sub_species="salmonicida"
                     /db_xref="taxon:382245"
                     /plasmid="pAsa1"
     gene            1014..1256
                     /gene="relB"
                     /locus_tag="ASA_pAsa101"
                     /old_locus_tag="pAsa1_01"
                     /db_xref="GeneID:1496836"
     CDS             1014..1256
                     /gene="relB"
                     /locus_tag="ASA_pAsa101"
                     /old_locus_tag="pAsa1_01"
                     /note="RelB; Qin prophage; bifunctional antitoxin of the
                     RelE-RelB toxin-antitoxin system/transcriptional
                     repressor"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional antitoxin/transcriptional repressor
                     RelB"
                     /protein_id="NP_861552.1"
                     /db_xref="GI:32453761"
                     /db_xref="GeneID:1496836"
                     /translation="MGTINLRIDDELKTRSYAVLERLGVTPSELLRQTLEYVASQERL
                     PFRTTVVTEDEEALLVLVRERLASPQKGIRVSLDDL"
     gene            1246..1533
                     /gene="relE"
                     /locus_tag="ASA_pAsa102"
                     /old_locus_tag="pAsa1_02"
                     /db_xref="GeneID:1496837"
     CDS             1246..1533
                     /gene="relE"
                     /locus_tag="ASA_pAsa102"
                     /old_locus_tag="pAsa1_02"
                     /note="RelE"
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="NP_861553.1"
                     /db_xref="GI:32453762"
                     /db_xref="GeneID:1496837"
                     /translation="MTFELEFDPRAWKEWKKLGDTVRQQFKKKLAEVLERPRVEANRL
                     HGLPDCYKIKLRGAGYRLVYQVQDDRVLVFVVAVGKREREQVYLDAGYRLE"
     gene            complement(1578..1649)
                     /locus_tag="ASA_pAsa1s1"
                     /old_locus_tag="pAsa1_s1"
                     /db_xref="GeneID:3722523"
     misc_RNA        complement(1578..1649)
                     /locus_tag="ASA_pAsa1s1"
                     /old_locus_tag="pAsa1_s1"
                     /product="misc_RNA"
                     /note="antisense RNA; regulator of rep; RNAI"
                     /db_xref="GeneID:3722523"
     gene            1717..2724
                     /gene="rep"
                     /locus_tag="ASA_pAsa103"
                     /old_locus_tag="pAsa1_03"
                     /db_xref="GeneID:1496838"
     CDS             1717..2724
                     /gene="rep"
                     /locus_tag="ASA_pAsa103"
                     /old_locus_tag="pAsa1_03"
                     /note="Rep; ColE2-type replication protein"
                     /codon_start=1
                     /transl_table=11
                     /product="replication primase"
                     /protein_id="NP_861554.1"
                     /db_xref="GI:32453763"
                     /db_xref="GeneID:1496838"
                     /translation="MVKPALQAAEFVERLPLRPYCTDDPALGLHIRPQATALAFRHIQ
                     HNPPPYVSCIVFDVDRKPYEQRREGYQEWRERGLPAPHWIAINPENGNYHLGYLLAAP
                     VARTNAARLKPLRYLAAIEHVLAKKLGADMGYVGLITKNPVHSDWWTVWHNHAPYSLD
                     YLAEFCPDADLAAYNRRSGKEASGLGRNVTVFDNVREWGYSAVREHWRPNGYDAWADA
                     VRAACESANAFGREQGGPLPHSEIKATAKSIARWIWRHLTPTSFADYVDRTHTPEIQA
                     RRGAKGGKVSKGGGRPSKAAELLPEVLRLKAMGYSNRDIAEDLQISAGSVSNYLRCDL
                     E"
     rep_origin      2736..2755
                     /note="oriV region"
     gene            complement(2840..3163)
                     /locus_tag="ASA_pAsa104"
                     /old_locus_tag="pAsa1_04"
                     /db_xref="GeneID:3722524"
     CDS             complement(2840..3163)
                     /locus_tag="ASA_pAsa104"
                     /old_locus_tag="pAsa1_04"
                     /note="Orf1"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="NP_861555.1"
                     /db_xref="GI:32453764"
                     /db_xref="GeneID:3722524"
                     /translation="MKDTTDQGTGDMFGIKRGRGRPKTGAAKSGAERQAAYRAKLADI
                     NVTVTINRELIDGLSVHLQAIRDGWSEVPLTPEQAGEILKALRTAELKQLRGQHPRPQ
                     AKPTA"
     oriT            3260..3278
                     /note="origin of transfer"
     gene            3372..3782
                     /gene="mobC"
                     /locus_tag="ASA_pAsa105"
                     /old_locus_tag="pAsa1_05"
                     /db_xref="GeneID:1496839"
     CDS             3372..3782
                     /gene="mobC"
                     /locus_tag="ASA_pAsa105"
                     /old_locus_tag="pAsa1_05"
                     /note="MobC"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861556.1"
                     /db_xref="GI:32453765"
                     /db_xref="GeneID:1496839"
                     /translation="MSKVQKITTVSVRIDKPTAAEWRERASASGLSLSDWIRGAVDAN
                     QQTNLPTPQRRPVRVRDTSNDADPALMRQLAGMSNNLNQIARALNECRLAGSSVQLVE
                     VLALLRSIEVQAGRLLPQLPPPPKPMAKGRDDAH"
     gene            3772..5424
                     /gene="mobA"
                     /locus_tag="ASA_pAsa106"
                     /old_locus_tag="pAsa1_06"
                     /db_xref="GeneID:1496840"
     CDS             3772..5424
                     /gene="mobA"
                     /locus_tag="ASA_pAsa106"
                     /old_locus_tag="pAsa1_06"
                     /note="MobA"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization relaxase"
                     /protein_id="NP_861557.1"
                     /db_xref="GI:32453766"
                     /db_xref="GeneID:1496840"
                     /translation="MLIKFLSHGTGSGARASAYLMGSHDHTGAPRAGVAVQRGDPVLF
                     AAVADSLPFQHRYASAVISWATEEAPTADEITAVLEDFEAVAFAGLEPEDVHLTAVQH
                     DEPDGGVHVHILIPRVHLGTGKSFNPAPPGHRKAFDAVRDKHNHEKGWARPDDPLRAR
                     LLRPDFEAFKDKSDKSAIKKQITEHLLGAAAQGVISNAAELQDYLQTSLGCEITRTGA
                     DYTSIKPEGYPKAIRLTGELYGSSWTAERTLEREAQTAARAGIGRGGEVSESGARQAQ
                     ERLRDACGRRAEYNRGRYPGRDKGIAQTDHRLDRPIEGGLGAGPERGQHPQADLQRLA
                     QSLAVRGMHLRGDRAGELGGIELDGQRGSAVEQRDQQRESHAGAASGRSELRPGGWQE
                     LHPRSDIGKNVRNAKWQDGGGDQVNDRIRTPTDRAAGADGAAAPQRDDGLAAAALRAT
                     DVFGELAKAVGEHLGRVRDALRQLTHRIRGLGEEHKERASQHGPEFGPDQPGYRSSTW
                     VIEQQCGRSQELAGELDQGAALIADRAAELRKEQRRDRGLSM"
     gene            4487..5020
                     /gene="mobB"
                     /locus_tag="ASA_pAsa107"
                     /old_locus_tag="pAsa1_07"
                     /db_xref="GeneID:1496841"
     CDS             4487..5020
                     /gene="mobB"
                     /locus_tag="ASA_pAsa107"
                     /old_locus_tag="pAsa1_07"
                     /note="MobB"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861558.1"
                     /db_xref="GI:32453767"
                     /db_xref="GeneID:1496841"
                     /translation="MDHLGQQKERLSERLKQRQEQELGEVEKLAKAVLDKLKKDLETR
                     VDGELNTTVDAIRDGIKGLPKQITDSTDPLKEVLEQARSEANTLKLISSVWLKALLFG
                     VCISVGIGLGSWGVLSWMGNEVQRLNSEISSAKATLAQLPAGVSFVREDGKSYILAPT
                     LGKTYETQSGKMAVEIK"
     gene            5021..5362
                     /gene="mobD"
                     /locus_tag="ASA_pAsa108"
                     /old_locus_tag="pAsa1_08"
                     /db_xref="GeneID:1496842"
     CDS             5021..5362
                     /gene="mobD"
                     /locus_tag="ASA_pAsa108"
                     /old_locus_tag="pAsa1_08"
                     /note="MobD; putative"
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="NP_861559.1"
                     /db_xref="GI:32453768"
                     /db_xref="GeneID:1496842"
                     /translation="MTELERLLIERLEQMELRHHNETMDLRQQLYAQQMSLESLQRQL
                     GSTLEGYETHCASLHTAFVALEKSTRSELASMGRSLDQTNRVTGAVLGSLNSSVAGVK
                     SSLESLIKAQR"
ORIGIN      
        1 tatatcgtca cagtgacaat aattaaggag atcgggtatg aaatcaggtc ggttgggtac
       61 tcctgacacg gtggcgcata ttcgccagtt tcccccggtt gggtacgaac tggcgagcta
      121 tcaaggctcc ccgtggctat gggccgatgg aaagccgctg atcgacctcc tcgatgcgac
      181 catcgactgg ctgatcgatg acgacggcag ccgctggatc gtcattgata ccgagtgcct
      241 gcgccagcat gtggcccact atgccctgtt gtccgccgat acatggcggc aacggctgct
      301 cgatgcaggt gtggaggtgg attaaggtgc gattgagtga ttggtttccg gggctgatgc
      361 tgtggcatac ggtggcggta ctggccgccg tgttcgcggc gaaagccctg ctgtactgtg
      421 ctgcggactg gttgggcctt gggccgctct acagcgcctt ggcggggcct tcttgggacg
      481 tggcggcatg gatagcgttg ttggccctgc tgctgcggtg gatgaaggtc tagcccgttc
      541 tgccgcgctg cgcttggcgg ccactatagc gggcgcgaca gactgcgcta gtcgtgtccg
      601 ctatagtggc tcgtaatttt gttcatctaa caaccataaa ggacttcgaa tggaaggcac
      661 acatctaaga cacattaatg atgaagagtt tagcccttta ctgcttgccg cttgcatcac
      721 gggtcacgat taccgcgacg cgtttaaaat attgcgagag caaggttgtt ctttgataaa
      781 taagaaattt tattatgatg acactcacaa aatctctact gggttagaga gcgtaaacca
      841 acaaaaacta actctaatgg cctatgtcag cataggagaa tacctagcta aaactggggg
      901 ggatgaaggt attcaacagc atcttaatga tattttttat caagtagttg agcttaaaaa
      961 tagagaatac gctaatatgt aagtacaaac gtacttacac ctggggtttg gatatgggga
     1021 cgattaacct tcgcatcgat gatgagctga aaacccgctc gtatgcggta cttgaacgac
     1081 ttggtgtaac tccatctgag ttactgcgac aaactctgga atatgtggcc tctcaggaac
     1141 gactgccttt tcggacgacc gtggtaacgg aggatgagga ggcattgctt gttcttgttc
     1201 gtgagcggct ggccagcccc cagaaaggga tcagggtgtc gctggatgac ctttgagctt
     1261 gagttcgacc cacgagcgtg gaaggaatgg aagaagttgg gcgatacagt tcgccagcag
     1321 ttcaaaaaga agctggcaga ggtgctggag cgacctaggg tagaggcgaa tcgtctgcat
     1381 gggctgccgg actgctacaa gattaagctg cgtggggctg ggtaccggtt ggtgtatcag
     1441 gtacaggatg atcgggtgct ggtcttcgtg gtcgcagtgg gtaaacgaga gcgggagcag
     1501 gtgtatctgg acgctggtta tcggctggaa taggtgaagt tgccgtgggg tggctagtac
     1561 ggtaacgtac tgtccagaaa agacaaaccc cgaatctctg tttagtcaac ttggcgggag
     1621 acaacagagg attcggggcc gttcactaag aacaggaaaa gcataacata tgcacgttct
     1681 cagacaaccc agcaccgcca ataagcgcgg tgatcgatgg taaaaccagc gcttcaagcc
     1741 gccgaattcg tcgaacggct ccccctccgc ccctactgca ccgacgatcc ggcgttgggt
     1801 ctgcatatcc gcccgcaggc aactgcgctg gccttccgtc acattcagca caacccaccg
     1861 ccgtatgtct cctgcattgt gtttgatgtg gacagaaagc cctatgagca gcggcgagaa
     1921 gggtatcaag agtggcgaga gcgtggcttg cctgcgccgc attggatcgc gatcaacccc
     1981 gaaaacggca actaccatct tggctatctg ctggcagcgc cagtggcgcg taccaatgca
     2041 gccaggctaa agccactgcg ctatctagcg gctatagagc acgtgctggc gaagaagctg
     2101 ggcgctgaca tggggtacgt tgggttgatc accaaaaacc ccgtacacag cgattggtgg
     2161 acggtatggc ataaccatgc gccctattcc ctcgactacc tcgccgagtt ctgcccagat
     2221 gcggatttag cggcctacaa ccgccgcagc ggcaaagagg ctagtgggct ggggcgtaac
     2281 gtcactgtgt tcgataacgt gcgggaatgg ggctacagcg cagtgcggga gcattggcgg
     2341 cccaatggat acgacgcatg ggcggatgcg gtgagagctg cctgtgagag cgccaatgcg
     2401 tttggacgcg agcagggcgg cccgttgccc catagtgaga tcaaggcaac tgccaagagt
     2461 atcgcccgtt ggatctggcg gcaccttacc ccgacctcgt ttgcggatta cgttgatcgg
     2521 acacataccc ccgagatcca agcgcggaga ggggctaagg gcggcaaggt atccaagggc
     2581 gggggcaggc ccagcaaagc ggcagagctg ctgccggaag tattgcggct caaagcaatg
     2641 ggctacagca accgcgatat agccgaggat ctgcaaatct cggctggcag tgtgtccaat
     2701 tacctccgtt gtgatcttga gtgatcaaaa acaagcctat atcagataac agcgcccgga
     2761 gggcgttcct ttatccctcc agtatttgag agagaggctc ggtgcgaggt gggcaagcgc
     2821 ccgaaccgta ggccgtccat caggccgtgg gtttggcctg tgggcggggg tgttgtccac
     2881 ggagctgttt tagttctgcc gttcttagcg ccttcaagat ctcgccagcc tgctctgggg
     2941 ttaatggtac ttctgaccat ccatccctga tcgcctgcaa atgtactgac agcccatcta
     3001 tcagctctct atttattgtc accgtgacgt taatatctgc caattttgca cggtaggctg
     3061 cttgccgttc tgccccgctt ttggctgctc ctgtctttgg cctaccccgt ccgcgcttga
     3121 tgccaaacat gtccccagta ccctgatccg tggtgtcctt cattgtgcta tttcctgtca
     3181 ctgtaacggt aattgattat gtgtcatcgt gacgataatt agcaagttgc cgcccctctc
     3241 tttttttgct gtactggtta ttagccagcc cacccgtatt acgttttagc ctgcttcgcg
     3301 gctcaacgta acacggggct ggtgaggggc tatgcccctt caatccccca cacaccacag
     3361 ccggaggccg cgtgtccaag gtccaaaaga taaccaccgt cagcgtcagg atcgacaagc
     3421 cgaccgccgc cgaatggcgt gaacgtgcta gcgcctctgg actcagtttg tctgactgga
     3481 tccggggggc ggtggatgcc aatcagcaga cgaatttgcc gacgccccag cgccgtcccg
     3541 tccgtgtccg cgataccagc aacgatgccg atccggcgtt gatgcgccag ttggccggga
     3601 tgagcaacaa cctcaaccag atagcccgag ctctgaacga atgccggttg gccggctcgt
     3661 cggtgcaact ggtggaagtg ctggccctgc tgcgcagcat agaggtacag gcgggacgcc
     3721 tgctgcccca gttgccgcca cccccaaagc ctatggccaa agggcgtgac gatgctcatt
     3781 aaattccttt cccacggtac gggcagcggt gcccgtgctt cggcctacct catgggctcg
     3841 cacgaccaca caggtgcacc acgggcaggt gtggcggtac agcggggtga tccggtgttg
     3901 tttgcggcgg tggccgactc gcttccgttc cagcatcgtt acgccagcgc agtgatctcg
     3961 tgggcgacgg aagaggcccc gaccgccgac gagattactg cggttttgga ggacttcgag
     4021 gcggtggcgt ttgctgggtt ggagccggaa gacgtccacc tgacggcggt ccagcacgac
     4081 gaaccggatg gcggggttca cgtccacatc ctgatccccc gtgttcacct gggcacaggc
     4141 aaatcgttca acccggcccc accaggtcac cgtaaagcct ttgatgcggt gagggataaa
     4201 cacaaccacg aaaagggctg ggcgcggcca gacgacccgc tccgggcgcg gttgctacgg
     4261 cccgactttg aggcgttcaa ggacaagagc gacaagagcg ccatcaaaaa gcagattacc
     4321 gaacacctac ttggtgccgc cgcgcagggg gtgatttcca atgcggcgga gctgcaggac
     4381 tacctacaaa catcgctcgg gtgcgaaatc acgcgcacag gggccgatta caccagcatc
     4441 aagcctgagg gctatcccaa agctatccgg ttaacagggg aactctatgg atcatcttgg
     4501 acagcagaaa gaacgcttga gcgagaggct caaacagcgg caagagcagg aattgggcga
     4561 ggtggagaag ttagcgaaag cggtgctcga caagctcaag aaagacttag agacgcgtgt
     4621 ggacggcgag ctgaatacaa ccgtggacgc tatccgggac gggataaagg gattgcccaa
     4681 acagatcacc gactcgaccg acccattgaa ggaggtcttg gagcaggccc ggagcgaggc
     4741 caacaccctc aagctgatct ccagcgtctg gctcaaagcc ttgctgttcg gggtatgcat
     4801 ctccgtgggg atcgggctgg ggagctgggg ggtattgagc tggatgggca acgaggttca
     4861 gcggttgaac agcgagatca gcagcgcgaa agccacgctg gcgcagcttc cggccggagt
     4921 gagcttcgtc cgggaggatg gcaagagtta catcctcgct ccgacattgg gaaaaacgta
     4981 cgaaacgcaa agtggcaaga tggcggtgga gatcaagtaa atgaccgaat tagaacgcct
     5041 actgatcgag cggctggagc agatggagct gcggcaccac aacgagacga tggacttgcg
     5101 gcagcagctc tacgcgcaac agatgtcttt ggagagcttg caaaggcagt tggggagcac
     5161 cttggaaggg tacgagacgc actgcgccag cttacacacc gcattcgtgg ccttggagaa
     5221 gagcacaagg agcgagctag ccagcatggg ccggagtttg gaccagacca accgggttac
     5281 cggagcagta cttgggtcat tgaacagcag tgtggccgga gtcaagagct cgctggagag
     5341 cttgatcaag gcgcagcgtt gatcgccgac agggctgcag agctgcgaaa agagcagcgg
     5401 agagatcgtg ggttgagcat gtga
//
