LOCUS       NC_009433              13873 bp    DNA     circular BCT 12-DEC-2007
DEFINITION  Rhodobacter sphaeroides ATCC 17025 plasmid pRSPA05, complete
            sequence.
ACCESSION   NC_009433
VERSION     NC_009433.1  GI:146280384
KEYWORDS    .
SOURCE      Rhodobacter sphaeroides ATCC 17025
  ORGANISM  Rhodobacter sphaeroides ATCC 17025
            Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales;
            Rhodobacteraceae; Rhodobacter.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
            Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F.,
            Land,M., Hauser,L., Kyrpides,N., Kim,E., Richardson,P.,
            Mackenzie,C., Choudhary,M., Donohue,T.J. and Kaplan,S.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Complete sequence of plasmid pRSPA05 of Rhodobacter sphaeroides
            ATCC 17025
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13873)
  CONSRTM   NCBI Genome Project
  TITLE     Direct Submission
  JOURNAL   Submitted (08-MAY-2007) National Center for Biotechnology
            Information, NIH, Bethesda, MD 20894, USA
REFERENCE   3  (bases 1 to 13873)
  AUTHORS   Copeland,A., Lucas,S., Lapidus,A., Barry,K., Detter,J.C., Glavina
            del Rio,T., Hammon,N., Israni,S., Dalin,E., Tice,H., Pitluck,S.,
            Chertkov,O., Brettin,T., Bruce,D., Han,C., Schmutz,J., Larimer,F.,
            Land,M., Hauser,L., Kyrpides,N., Kim,E. and Richardson,P.
  CONSRTM   US DOE Joint Genome Institute
  TITLE     Direct Submission
  JOURNAL   Submitted (10-APR-2007) US DOE Joint Genome Institute, 2800
            Mitchell Drive B100, Walnut Creek, CA 94598-1698, USA
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from CP000666.
            URL -- http://www.jgi.doe.gov
            JGI Project ID: 4000699
            Source DNA and bacteria available from Samuel Kaplan
            (Samuel.Kaplan@uth.tmc.edu)
            Contacts: Paul Richardson (Microbes@cuba.jgi-psf.org)
                      Samuel Kaplan (Samuel.Kaplan@uth.tmc.edu)
                      Ronald C. Mackenzie (Ronald.C.Mackenzie@uth.tmc.edu)
            Quality assurance done by JGI-Stanford
            Annotation done by JGI-ORNL and JGI-PGF
            Finishing done by JGI-LANL
            Finished microbial genomes have been curated to close all gaps with
            greater than 98% coverage of at least two independent clones. Each
            base pair has a minimum q (quality) value of 30 and the total error
            rate is less than one per 50000.
            The JGI and collaborators endorse the principles for the
            distribution and use of large scale sequencing data adopted by the
            larger genome sequencing community and urge users of this data to
            follow them. It is our intention to publish the work of this
            project in a timely fashion and we welcome collaborative
            interaction on the project and analysis.
            (http://www.genome.gov/page.cfm?pageID=10506376).
            COMPLETENESS: full length.
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Rhodobacter sphaeroides ATCC 17025"
                     /mol_type="genomic DNA"
                     /strain="ATCC 17025"
                     /db_xref="ATCC:17025"
                     /db_xref="taxon:349102"
                     /plasmid="pRSPA05"
     gene            complement(144..824)
                     /locus_tag="Rsph17025_4391"
                     /db_xref="GeneID:5086572"
     CDS             complement(144..824)
                     /locus_tag="Rsph17025_4391"
                     /note="PFAM: SMF family protein"
                     /codon_start=1
                     /transl_table=11
                     /product="SMF family protein"
                     /protein_id="YP_001170539.1"
                     /db_xref="InterPro:IPR003488"
                     /db_xref="GI:146280385"
                     /db_xref="GeneID:5086572"
                     /translation="MDTAFRTDEVLGFLGPFEKKNAPEALYMAGDAALLKTGTRVAVV
                     GSRKSSENGEKRARALVRALVEQGITVVSGLAEGIDTVAHETAIEAGGRTIAVLGTPL
                     SQAYPAKNRRLLDIIKKDHLAISQFPEGYPTKGENFPRRNRTMALICDATIVVEAGEK
                     SGTRHQGWEAIRLGRLVYLLENVVNKPGMTWPKELIAYGAQVLRREDLDNLKYDIPNF
                     TAGLEVAF"
     gene            2186..2581
                     /locus_tag="Rsph17025_4392"
                     /db_xref="GeneID:5086573"
     CDS             2186..2581
                     /locus_tag="Rsph17025_4392"
                     /note="PFAM: transposase IS3/IS911 family protein"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase IS3/IS911 family protein"
                     /protein_id="YP_001170540.1"
                     /db_xref="InterPro:IPR002514"
                     /db_xref="GI:146280386"
                     /db_xref="GeneID:5086573"
                     /translation="MPEHVLMSEIEIITDGGRRRRWTAAEKVRIVEETLDDRASISVV
                     ARRNGVAPNLLYRWRRLMLEGGSVAVAEDDDVTSNRVVRQMEDRIRELERQLGRKTLE
                     VEILREALDKSRSKKPTLLARSPLRDDFQ"
     gene            2578..3411
                     /locus_tag="Rsph17025_4393"
                     /db_xref="GeneID:5086574"
     CDS             2578..3411
                     /locus_tag="Rsph17025_4393"
                     /note="PFAM: Integrase, catalytic region"
                     /codon_start=1
                     /transl_table=11
                     /product="Integrase, catalytic region"
                     /protein_id="YP_001170541.1"
                     /db_xref="InterPro:IPR001584"
                     /db_xref="GI:146280387"
                     /db_xref="GeneID:5086574"
                     /translation="MKAVAETLGVARSNLIERLQGKTKPRRRYQKVQDAAVVPRITAL
                     VSARPTYGYRRITAILNRQLRSEGLAPVNHKRVYRIMQAHNLLLARKYSERPEHVHDG
                     KVIVMRSNLRWCSDGFEFTCWNGDIVRGAFIIDAHDREIIAWRAVVNAGISGSDIRDI
                     MLEAVERRFGAYRAPSVIEMLSDNGPPYIAKDTQIFARQLGLKPCFTPVQSPQSNGIS
                     EAFVKTLKRDYVQVTPLPHAQTALGLIGAWIEDYNENHPHSGLKMRSPREFIAAQTAT
                     A"
     gene            3715..4311
                     /locus_tag="Rsph17025_4394"
                     /db_xref="GeneID:5086575"
     CDS             3715..4311
                     /locus_tag="Rsph17025_4394"
                     /note="PFAM: Resolvase, N-terminal domain"
                     /codon_start=1
                     /transl_table=11
                     /product="Resolvase, N-terminal domain"
                     /protein_id="YP_001170542.1"
                     /db_xref="InterPro:IPR006119"
                     /db_xref="GI:146280388"
                     /db_xref="GeneID:5086575"
                     /translation="MDAQRRDIDLFLAQYAEQPWEIIAELEDIQSGADSRRPALTEAV
                     ELARKTGATILVSKLDRLSRKVSFIATLLDDKRVQFRVASMPQADKFQLHIYAALAEQ
                     EREFISLRTKAALAEAKAKGKQLGGLRDATGKRNEAVQREAAERAETVAPLILPMRAG
                     GKSLREIADALNETGVRTARNGNWTASQVSRTLTRLNA"
     gene            4366..4674
                     /locus_tag="Rsph17025_4395"
                     /db_xref="GeneID:5086576"
     CDS             4366..4674
                     /locus_tag="Rsph17025_4395"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001170543.1"
                     /db_xref="GI:146280389"
                     /db_xref="GeneID:5086576"
                     /translation="MRCALLLGSVLALAATELNAGSVEDVMEDYRKALAPLLAEVRSN
                     PLTAEGYWLEMRNIGDEWEKMILVFGYAGAGDEAACAEVLRLVAGENLERDYRCVPVN
                     "
     sig_peptide     4366..4428
                     /locus_tag="Rsph17025_4395"
                     /product="hypothetical protein"
                     /note="Signal predicted by SignalP 2.0 HMM (Signal peptide
                     probability 0.986) with cleavage site probability 0.757 at
                     residue 21"
     gene            complement(4761..5363)
                     /locus_tag="Rsph17025_4396"
                     /db_xref="GeneID:5086577"
     CDS             complement(4761..5363)
                     /locus_tag="Rsph17025_4396"
                     /note="PFAM: plasmid pRiA4b ORF-3 family protein"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="YP_001170544.1"
                     /db_xref="InterPro:IPR012912"
                     /db_xref="GI:146280390"
                     /db_xref="GeneID:5086577"
                     /translation="MTLVARLKVTLSDVEPEVLRRFDVPLKIRLNRLHEVIQAAMGWS
                     DTHLYEFRAGGVGWGVADPDGFHDGPLPASKTSLLDVIEDTGTKTIHYIYDFGDNWHH
                     VIRVEKIDDALPGADYPRLVRAIGACPPEDVGGAPGYAEFLEARADPNHEAHADMMDL
                     HGGTFDPDDAGIGRILDSFERLAKKWAPRPRKPKTTSRPL"
     gene            complement(5360..6973)
                     /locus_tag="Rsph17025_4397"
                     /db_xref="GeneID:5086578"
     CDS             complement(5360..6973)
                     /locus_tag="Rsph17025_4397"
                     /note="PFAM: transposase IS66"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase IS66"
                     /protein_id="YP_001170545.1"
                     /db_xref="InterPro:IPR004291"
                     /db_xref="GI:146280391"
                     /db_xref="GeneID:5086578"
                     /translation="MVEAAMSDPACELEKLRAELAATKAELAQARAVVSTSEAMIAGL
                     KLEIALLRREHYGRSAERTARLIDQLELQLEELVTAAAEDRIRVEKTTKVAAFERRKP
                     VRKPFPEHLPRERVVVPAPTSCSCCGSDRIAKMGEDVTETLEVVPRQWKVIQTVREKF
                     TCRACEKISQAPAPFHPIPRGWAGPSLLAMIVFEKFGQHQPLNRQAERYAREGVDLSL
                     STLADQIGAVAELLKPLHDLIEAHVLAASRLHGDDTTVPLLARGGTKTARLWTYVRDD
                     RPFSGTAPPAVVFRFSRDRAGEHPAEHLTGWKGILQADAYAGYNQLYRGDRKPGPVLP
                     ALCWSHARRKFFELADVEGNIRKGKDAKEISPIAFEAVRRIDAIFEIERKLNGQDPAA
                     RLAARQERSAPLVEDLERWLRTERALLSKHAKVAKAIDYLLSPDHWPGFTRFLSDGRV
                     CLSNNCAERALRGVALGRKSWLFAGSERGGQRAAFMYSLICTAKLNGIDPQAWLADAI
                     ARISDLPVSRLPELLPWHWCNPAHPRIAA"
     gene            complement(7016..7366)
                     /locus_tag="Rsph17025_4398"
                     /db_xref="GeneID:5086579"
     CDS             complement(7016..7366)
                     /locus_tag="Rsph17025_4398"
                     /note="PFAM: IS66 Orf2 family protein"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 Orf2 family protein"
                     /protein_id="YP_001170546.1"
                     /db_xref="InterPro:IPR008878"
                     /db_xref="GI:146280392"
                     /db_xref="GeneID:5086579"
                     /translation="MIAFPAGTRVWIAGGVTDMRCGMNSLALKIQQGLGRDPHGGEIF
                     CFRGRKADMVKLLWFDGVGVSLYAKRLEAGKFVWPSSTTGEAVAISAAQLGYLLDGID
                     WRNPRWTQRPKKMG"
     gene            complement(7363..7770)
                     /locus_tag="Rsph17025_4399"
                     /db_xref="GeneID:5086580"
     CDS             complement(7363..7770)
                     /locus_tag="Rsph17025_4399"
                     /note="PFAM: transposase IS3/IS911 family protein"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase IS3/IS911 family protein"
                     /protein_id="YP_001170547.1"
                     /db_xref="InterPro:IPR002514"
                     /db_xref="GI:146280393"
                     /db_xref="GeneID:5086580"
                     /translation="MSCAKVISQFEVFAAGELGRRRTWSDAEKIRIVEESYLGHRQGS
                     VVARRHGISRALLTQWRRAWHKGLLDGGRPVFTPVEIGPDLRDRQREAPAMQGEDAHA
                     RIEITLANGRRLAVSADIEAVALTRLVQVLERA"
     gene            10009..10704
                     /locus_tag="Rsph17025_4400"
                     /db_xref="GeneID:5086581"
     CDS             10009..10704
                     /locus_tag="Rsph17025_4400"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001170548.1"
                     /db_xref="GI:146280394"
                     /db_xref="GeneID:5086581"
                     /translation="MKVARNWVKLPTGWIAAGGLKAFAWGKEGGSNQTAALMVLLAIA
                     HRADDENGLVRLPYDELQWATHLSRQKVADGLDVLGSRKIILRGSQGRSTLQLASYDP
                     KQGWAMLPASRLYSSDGIIAFADFHLRKKVELDALKAYLAFVARRDREQNRAFITYEQ
                     IHDYAGIPEGRIKPAISLLVVQGLVVVEHMERSGGLGSSHGYRLSYLHTRNHMGTNGR
                     AHFGETQAGDDLF"
     sig_peptide     10009..10083
                     /locus_tag="Rsph17025_4400"
                     /product="hypothetical protein"
                     /note="Signal predicted by SignalP 2.0 HMM (Signal peptide
                     probability 0.628) with cleavage site probability 0.612 at
                     residue 25"
     gene            11110..12153
                     /locus_tag="Rsph17025_4401"
                     /db_xref="GeneID:5086582"
     CDS             11110..12153
                     /locus_tag="Rsph17025_4401"
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid replication initiator protein-like
                     protein"
                     /protein_id="YP_001170549.1"
                     /db_xref="GI:146280395"
                     /db_xref="GeneID:5086582"
                     /translation="MGEVTNERSPLLPDRHPQRDLFVCDIVDAVPKGDMSSMEHPVFS
                     LSTKPDMRPRRYERGGNWIEISPSRYGLATVHDRDVLIYCISQCMGALNEGRKVARTL
                     RFKAHDLLVATNRQTSGRGYELLKDALRRLQGTQIETNLRQGGKEYFRVFGLIDSAEV
                     VRETRDGRMLDVEITLSDWVFDAIENNHVLTLNRRYFFLRKPLERRLYELARKHCGQQ
                     QEWRIGLDTLREKCGSGSSSKEFKRLVGKIIDDDAQHDYMPDYALTLDGEIVVVRPKK
                     EAAQIPLPLASSVSLRINPDSYDKARQLAPGWDVHVLESEWRSWVMDKSIAVKTPDAH
                     FLGFCKKRGPYGR"
     gene            12270..12923
                     /locus_tag="Rsph17025_4402"
                     /db_xref="GeneID:5086583"
     CDS             12270..12923
                     /locus_tag="Rsph17025_4402"
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="YP_001170550.1"
                     /db_xref="GI:146280396"
                     /db_xref="GeneID:5086583"
                     /translation="MTYIIGMVSQKGGVGKSTLARMMAREFVSGGLSAKIADLDTQQQ
                     TCTNWAGRRAENAVTPELQVQSFATVKTALEDAARFDALILDGKPNASEQTLEIAQAA
                     DLIVIPSGQTVDDLHPGVVLAHSLRKKGIAPMKIAFAMFKTTGSDRENHAARQYLIDA
                     GYAVLDGDVPVSTAYGSASDTGKAITETSFRTLNSRAAMLAQSVIDKLAELQERQVA"
ORIGIN      
        1 cgttttcttc ccgcaggtgc gggatggcgc tttccagaac gttaatcttg cctgccttta
       61 tagccccgca tattctgcgc gacctatccg ataattcaga cgtgccgcgt ggagagtaat
      121 ttgcaaatgt accgtagtca taatcagaag gcaacttcca agcccgccgt aaagtttggt
      181 atatcgtact tcagattatc taaatcttcg cgccgcaaaa cttgcgcgcc ataagcaatg
      241 agttctttcg gccaagtcat gccaggctta ttcaccacgt tttctagaag atagacaagg
      301 cgacccaagc ggatagcctc ccatccctga tgccgagttc cgcttttttc accggcctct
      361 actacaattg tggcgtcgca aataagagcc atggtgcgat tacggcgggg gaaattttcc
      421 cccttcgtcg gataaccttc cggaaactgc gaaatcgcaa gatgatcttt cttgatgatg
      481 tcgagcaggc ggcggttctt cgcgggataa gcctgcgaaa gcggcgttcc aagcaccgct
      541 atggtccgac cgcccgcctc tatcgcggtc tcgtgcgcta ccgtgtcgat tccttcggca
      601 aggccactga caacggtgat gccttgttcc acaagcgccc ggacaagagc gcgggcacgc
      661 ttttctccat tctccgatga ctttcgcgag ccaacgaccg caacgcgcgt tcctgtcttc
      721 aggagcgcgg cgtctccagc catatagagc gcctcgggag cgttcttctt ctcgaacggc
      781 cctagaaagc ccaagacctc atctgtacga aatgcagtgt ccatacccta ctttgctctt
      841 ggcagtgcat tgtattaact tccagttttc ttatgttata caataccttg ggctctgcca
      901 agaccttaag tgatactggc agctctcact agagcagcat taccgttagg gtgacaccgg
      961 tgcccgcgcc gccggaaggt ggcgataaaa acattgcggt cgagatgcct agggggttgg
     1021 ccatgtccac gactgtgaag gacagtcagc gccagcatgg ccttaggcgc cgtcaccgcg
     1081 gcgcggcgag gtcactaccg ggtcaggacg aattgttcgc actcctcgac tgagccaagg
     1141 ccgccgagtg cgtctcgatc tggcttatga gcgtgctcgg gcccttacgc ttgcgccagc
     1201 aactttgcca cacaagggag tattggcgcg catattagga cagtagtcga tagggcaaat
     1261 tctctctcag caaaagagat caacggtact tccttagcgt ttccccctta ggggttcacc
     1321 gagctttttt gaatgcctga gagattcagg tcgtcacgcc aaatatctcc agcttcgtca
     1381 tctctccatc cttgtcttgg atgtagaact taccgccact ttcggacttc aagaggaagt
     1441 actctagaag acgaagggca tctttggtga catcggtcac cgtgtcggct cccatcatag
     1501 ccaccagtcg gtcaatcctc gccttatcct tttcggagag aaccacgttc agacggtgtg
     1561 cttttgcgcg aggagtatca ggccccgcgc gttgtagatt gctcatatca ctcccttttc
     1621 gctgtgccgc caaagtagtg ccagaagatg gcgccaacga ccggaccagc gacggcccaa
     1681 actcgctcca gctcatcaaa ggtcccgtcg cggaacccga taacgccggc aacgatgata
     1741 gaaagagcgg taaagcccaa ggtgccatag gtaaccctgg tcctagtatt accctcatga
     1801 actcgagcca ccgcatcgat cacatcagcg gaagcttcaa ccggcttact cagcgacgca
     1861 tcgagactct cgaattcgtc accaatgatt actctcttct tcaccgcccc catccctcct
     1921 gggatcagag ccgccaactc tttcttccac ggtttttcaa acgaaggcag ctaacgactc
     1981 aactcttcgg tcgactttgc cgcggaagaa agccacccca tgagggaggt ccttcgtttg
     2041 gtagtgaggc ttacctcact gatagtacaa agttcatttt cggcttttcc tcggtttccc
     2101 cctcccctgt gggaagacaa agggagggct gaactggccc ccgtttcgac cggacacttg
     2161 ggcataacca tggaggctca ggcatatgcc tgagcacgtg cttatgtctg agatagagat
     2221 catcaccgat ggcggccgtc ggcgccgctg gactgccgcc gagaaggtgc ggatcgtcga
     2281 ggagacgctg gacgaccggg ccagcatctc ggtcgtggcg cgccgcaatg gtgtggcgcc
     2341 aaacctgttg tatcgttggc ggcggctcat gctcgaaggg gggagtgtcg ccgtggcgga
     2401 agacgatgac gtcaccagca acagggtcgt ccgccagatg gaggaccgca tccgcgaact
     2461 tgaacgtcag ctcggccgca agacgctgga ggtcgagatc ctgcgcgagg ccctggacaa
     2521 gtcacgctca aaaaaaccga ccttgctcgc gcggtcgccg ctgagggacg atttccagtg
     2581 aaggccgtgg cggaaaccct gggcgtggcc cggtccaacc tgatcgagcg gctgcaaggc
     2641 aagacaaagc cgcgtcggcg ctatcagaag gtgcaagacg cggcggtggt gccaaggatc
     2701 acggcgctcg tgtcggcgcg cccgacctat ggctaccggc ggatcaccgc gatcctgaac
     2761 cgccagttgc gctcggaagg cttggcaccg gtcaatcaca agcgggtcta ccggatcatg
     2821 caggcccata acctgctgct ggcccggaaa tattccgaac gccccgagca tgttcacgac
     2881 ggcaaggtga tcgtcatgcg ctccaacctg cgctggtgct cggacgggtt cgagttcacc
     2941 tgctggaacg gcgacatcgt ccgcggcgct ttcatcatcg atgcccatga tcgggaaatc
     3001 atcgcatggc gcgccgtggt gaacgccggg atcagcggct ccgacatccg agacatcatg
     3061 ctcgaagccg tcgaacgccg cttcggcgca taccgcgccc catcggttat cgagatgctc
     3121 tccgacaacg ggccgcccta catcgcgaag gacacccaga tcttcgcccg ccaactcggt
     3181 ctgaagccct gcttcacccc ggtccagagc ccgcagagca acggcatctc ggaggctttc
     3241 gtgaagacgc tgaagcgcga ctacgtccag gtgacgccat tgccccacgc acagaccgct
     3301 ctcggattga tcggagcctg gatcgaggat tacaacgaaa accacccgca ctcagggctg
     3361 aaaatgcgct cgccccgcga gttcatcgca gcccaaaccg caaccgcctg agtgtccggt
     3421 gaaacggggg caaaaccaag ggctttccgc ctagtttttg ggactctttc aaaaaagtgg
     3481 ttgtacccgc ccccgtggga gagatggacg ggcaaggcgg aatctgcacc actatgtgcg
     3541 cgatgtcaac catagttgcg catcttatgc tcatgtatca ctcacaacga acgttgacgt
     3601 ttgcaacgca accttctacg acaggggcaa ccctgatcga ggaggacagt cattcagcag
     3661 ttcatcatct accgacgcgt cagcaccgag gatcagggac gctctggtca tggcatggat
     3721 gcccagcggc gggacatcga cctattcctg gcccaatacg ccgagcagcc gtgggagatc
     3781 atcgccgagc tggaggacat ccagtccggt gctgacagcc gccgtcctgc gctgaccgag
     3841 gcggtggagc ttgcacggaa gaccggggct acgatcctcg tctcaaagct cgaccggctg
     3901 tcgcggaagg tgtcgttcat cgcgaccctg ctggacgaca agcgggtaca gttccgggtc
     3961 gcctcgatgc cccaggcgga caagttccag cttcacatct acgcggcgct ggccgagcag
     4021 gagcgggagt tcatctcgct gcggaccaag gccgctctgg ccgaagccaa ggcgaagggg
     4081 aagcagctcg gcgggctgcg tgatgccacc gggaagcgga acgaggcagt gcagcgtgaa
     4141 gcggccgagc gggccgagac ggtggcaccg ctgatcctgc cgatgcgggc gggaggcaag
     4201 agcctgcggg agatcgcgga cgctttgaac gagacgggtg tcaggactgc cagaaacggg
     4261 aactggaccg catcccaggt ctctcgcaca ttgaccaggc tgaatgcgtg acccccagac
     4321 gcttctcaag ctcatgacaa gagccaacct acaaggagac tttgtttgcg gtgcgccctt
     4381 cttcttggct ctgtgttggc tttggcggca acagagctga atgccggttc agttgaggat
     4441 gtgatggagg actaccggaa agccctcgca cctttgcttg ccgaggttcg atctaacccc
     4501 ctcaccgccg aaggctactg gctggagatg cgcaacatcg gagacgagtg ggagaagatg
     4561 attctcgtct ttggctacgc aggtgcaggt gatgaggcag cctgtgctga ggttttgagg
     4621 cttgttgccg gtgagaacct ggagagggac taccgctgcg taccagtgaa ctgatcgtcc
     4681 atcacccaaa tgcccaagaa ggtgccgcgc ctcagagctt ttcgtaagca tccggcaaag
     4741 gccgcgggcc ggccggtctc tcagagcggc ctgctggttg tcttcggctt ccggggcctc
     4801 ggcgcccatt tcttcgccag gcgctcgaag ctgtcgagga tgcggccgat gccggcgtcg
     4861 tccgggtcga acgtgccgcc atggaggtcc atcatgtcgg cgtgagcttc atggttcgga
     4921 tcggcgcgtg cctcgaggaa ttcggcatag ccgggggcac cccccacgtc ctcgggagga
     4981 caggcgccga tggcacggac aaggcgcggg tagtcggcgc cggggagagc gtcgtcgatc
     5041 ttctcgaccc tgatcacatg gtgccagttg tcgccgaagt cgtagatgta gtggatcgtc
     5101 ttcgtgccgg tgtcctcgat gacatcgagc aggctggtct tgctggcggg tagagggccg
     5161 tcgtggaagc cgtcgggatc ggcgacaccc cagccgacgc ctcctgcgcg gaactcgtag
     5221 aggtgcgtgt cgctccagcc catggcggcc tggatcacct cgtgcagccg gttaagccgg
     5281 atcttcagcg gcacgtcgaa gcggcgcagc acctccggct cgacgtccga cagggtgacc
     5341 ttgaggcggg cgaccagcgt catgcggcga tcctgggatg ggctgggttg caccagtgcc
     5401 acggcagcag ttcgggcagg cgtgagaccg gcaggtcgga gatccgggca atggcgtccg
     5461 cgagccatgc ctgcgggtcg atgccgttga gcttggcggt gcagatgagg gaatacatga
     5521 aggcggcgcg ctgtccgccg cgctcggaac cggcgaagag ccagctcttg cggccgaggg
     5581 ccactccccg cagcgcccgc tcggcgcaat tgttcgacag gcagacccgg ccgtcggaca
     5641 ggaaccgggt gaagcccggc cagtgatcgg gcgagagcag gtagtcgatc gccttggcca
     5701 ctttggcgtg cttcgacagg agcgcgcgtt cggtccggag ccagcgctcc agatcctcga
     5761 ccagcggggc cgagcgttcc tgtcgtgctg cgagacgggc ggcgggatcc tgcccgttca
     5821 gcttgcgctc gatctcgaag atcgcgtcga tgcgcctgac cgcctcgaag gcgatcgggg
     5881 agatttcctt cgcatctttg cccttgcgga tgttcccctc gacatcggcc agctcgaaga
     5941 acttgcgccg ggcatggctc caacaaagcg ccggcaggac cggccccggc ttccggtcgc
     6001 cgcggtagag ctggttgtag ccggcgtaag cgtcggcctg caggatgccc ttccaccccg
     6061 tcaggtgctc ggccggatgc tccccggccc ggtcacggga gaagcggaag accaccgccg
     6121 gcggggcggt gccgctgaag gggcggtcgt cgcgaacata ggtccagagc cgcgccgtct
     6181 tcgtgccgcc ccgggcgagc agcggcaccg tggtgtcgtc gccatgcagg cgggaagcgg
     6241 caaggacgtg cgcctcgatc aggtcatgga gcggcttcag cagttccgcc acggcgccga
     6301 tctggtcggc gagcgtcgaa aggctcaggt ccacgccctc gcgggcatac cgctcggcct
     6361 ggcggttcaa cggctgatgc tggccgaact tctcgaagac gatcatggcc agcaggctcg
     6421 ggccggccca gccgcgcggg atcgggtgga agggcgccgg cgcctggctg atcttctcgc
     6481 aggcccggca agtgaacttc tcccgaaccg tctggatcac cttccactgc cgcggcacca
     6541 cctcgagcgt ctcggtgacg tcttccccca tcttcgcgat gcggtccgat ccgcagcagg
     6601 aacaagacgt cggcgccgga accaccacgc gctcgcgcgg cagatgctcg gggaagggtt
     6661 tgcgaaccgg cttccggcgc tcgaaggccg cgaccttcgt cgtcttctcc acacggatcc
     6721 tgtcctcggc ggcggcggtc accagctcct cgagctgcag ttccagctgg tcgatcagcc
     6781 tggcggtgcg ttcggcgctg cgcccgtagt gctcgcgccg gagcagcgcg atctcgagct
     6841 tgagccccgc gatcatcgcc tccgaggtcg agaccaccgc gcgcgcctgc gccagttcgg
     6901 ccttcgtggc cgccagttcg gcgcgcagtt tctcgagttc gcaagcggga tcggacatgg
     6961 ccgcttctac catgcacagc cacggaagcc aacaattaca gatgcttccg cgacatcagc
     7021 ccatcttctt cggcctctgg gtccagcgcg gattgcgcca gtcaattccg tccagcagat
     7081 agccgagctg agctgcggat atcgcaacgg cctcgcccgt ggtgctcgac ggccagacaa
     7141 acttgcccgc ctcgaggcgc ttcgcataga gcgacacgcc caccccgtcg aaccacagca
     7201 gcttcaccat gtccgccttg cggccgcgga agcagaagat ctcgccgcca tgcgggtcgc
     7261 ggccgagccc ctgctggatc ttgagcgcga gcgagttcat cccgcaccgc atgtccgtga
     7321 cgccgccggc gatccacacc cgcgttcccg ctggaaaggc gatcatgcgc gttcgagcac
     7381 ctgcacgagg cgcgtcagcg ccaccgcctc gatgtctgcc gacacggcga ggcgtcggcc
     7441 gttcgccagc gtgatttcga tccgcgcgtg ggcatcctct ccttgcatgg ccggtgcctc
     7501 ccgctgtcgg tcacgaagat ctgggccgat ctccaccggc gtgaaaaccg gacgtccgcc
     7561 gtccagcagc cccttgtgcc aggcgcgccg ccattgcgtc agcagcgcgc gcgagatgcc
     7621 gtgccggcgt gcgaccaccg agccctgccg gtgaccgaga tagctctctt cgacgatgcg
     7681 gatcttctcc gcgtccgacc aggtccggcg gcggccaagc tcgcccgccg cgaagacctc
     7741 aaattgtgaa atgaccttag cgcatgacat acgtcatgac gtaagtcata aaaccaggcc
     7801 ccggcaggcg gccctcggcg gagccttaca gcttttcgtt tacatcgaag ccttcagcgg
     7861 ttcagcacca acggccagag accctctgat tacaaagcag atgacctggt tcgatgggtg
     7921 tggcggaacc gttaggtgca gcaggtttag gttctgacgc cgacccgctc cgacaccgaa
     7981 ctcccggcgc cagccgtgat aggtgaactg cgtcacgctg atcaaccgca cggcctccgc
     8041 caccgaccgg ccctgccaac cgtctcacgg agttaatgac gcctcgatct tcggacagta
     8101 cttcgattta attttcttgg cgcctgtcgc gctgagcagc gatgtcaacg aaaacgaaaa
     8161 tgcttaaacc gacgtagtgg attggagaca ttttttatga tgtcatcaat tacgaaatct
     8221 aaattcgatt taaaatcaga atttaattca tcgtatggca gtacacctga attaataatt
     8281 atattagagt tctgattgct gacacatagt gcgttttctg ccttggcgat atatccaata
     8341 aagtcagaat atggatgcat ttcatactta ttaaagccat tgcttttctt cgagtgatca
     8401 taaggccgaa tgtaaaccgg agtgcttcct ccgacgaagc ttgcgacgcc ataaaccgac
     8461 ttcatttttt catctaagtg tttgataata gaaggtatac gtccgattga agactttgga
     8521 acgatcagcg ccgcgtggaa gtgcggcctg gagggagtta tgacaccgcg aaccttggtg
     8581 gtcccttcga agtcagcgca ccatgccaac gcgggacgcc gatgcagcgg gatatgttca
     8641 tccagattgt aagcgaggta tcgctctgcg agctggtata gatggcctgc aacgaaatcg
     8701 acattcgtaa tgttcttgac atggactgta atgaatatgg tcctaatatc aatttctgtc
     8761 gcatccttca tgtgacacaa catcttcgca attatccaat tcgagttatg gatagcaaga
     8821 tttgatttcg atgtctcttg agcttgatac gctcggctgc tacaattcat cttattccct
     8881 ttatctatta ttgacaatac tttcccaacc tcgcgccagg cgcaagatcg gctataataa
     8941 tgatttcaaa atttcagcca tagctccgca gccgacggaa tataggtttc cgtcaactac
     9001 gatgctatga ttggggttca ttcgcgcttg gactccggaa tccaaacttg cggccgcaat
     9061 gcgaatatca taatatatac tggaaatctt cgcccaagtg attcagaaga atcacaccgc
     9121 aggggccgtc attcagtggc ttcagtatat atatctccta cggataagtc aatcttataa
     9181 tagcgttatt gagcgtgtgt tgcaaaaaat cgcctattgc aagctacggt atgcttcgac
     9241 gtatgttgct gatatgagag gttagatgtc tataagatcg agaatttcta cgcgcttgga
     9301 tgaccttgaa cataggctga agtcggatga tcacttaaaa ttggatggcg ttggctccgt
     9361 cgagctgttg atcagcagca tatctaaatt ctggagtgtt ctgaccgagg aagagcggga
     9421 cttcgttgcc gcggcgcgct tcgcggttga agagcaggtt tcgtggactg gtgcgtcagc
     9481 ttgagctgcc cgtgtagtag acgcgctcga atgtactgat cgacgctaga tgaccgaggt
     9541 agggcgtggc gaccgcccgc agggtgatct atcttggcgc ccacgcgggc cgcaggccct
     9601 acttcttctt gaaggaacgg caagcagccg aaggctgcag gaactcccca agagtggcgt
     9661 tagcaataac agccacctgt tcagtctatg aacgacatgc catagaccga atcagggtct
     9721 caaaaagatt gatgttttat ctatcaagtt cctgttttta atagataaaa atcatatcct
     9781 tccgttgaaa gatcgtgagt tgtatctaga atagaacctt ggaggaggca aaatccgcat
     9841 catcgaagct ttcacgacga ggcaaaacat gacggagctt tctgaaactg ccgggatggc
     9901 actgtcggca cccaccacga cgctcgcgct gatccttgcg atcctcgcag tcggcatctg
     9961 cggctttgcg ctgttcctgg tgtaccgggt gctgcacggg aggcctgaat gaaagttgct
    10021 cggaactggg tgaagctgcc cactggctgg atcgctgccg gtggactgaa agcatttgct
    10081 tgggggaaag aaggcggatc gaaccagacg gcggcactta tggtgctgct ggcgatcgcg
    10141 catcgtgccg acgatgaaaa cgggctggtg cggttgcctt acgacgaact gcaatgggcc
    10201 acgcatctga gcaggcagaa ggtggccgat gggctcgacg tacttggttc gcgtaagatt
    10261 atcctacgcg gatcgcaggg gcgtagcacg ttgcagcttg cgagttacga cccgaagcag
    10321 ggctgggcga tgctaccggc aagtcgcctt tactcttctg acggaattat cgcttttgcg
    10381 gattttcatc tgcgcaagaa agtcgagctg gacgcgctca aggcttacct tgccttcgtt
    10441 gcccgccgtg atcgggagca gaaccgcgcc ttcataacct acgagcaaat acacgactat
    10501 gcgggtatcc ccgaaggaag gataaagcca gcgatcagcc tgctagtcgt gcaggggctt
    10561 gtggtcgttg agcacatgga gcgcagcggc gggctcgggt cgtcgcatgg ctatcggctt
    10621 tcctatctcc acacgcgcaa ccacatggga acaaacggac gcgcacactt tggcgagaca
    10681 caagctggag acgatctttt ctgacggcac atggcggtac cccgctagat tgctggcgcc
    10741 gcagcttctt gccctgcgcc tgaccggctg cgagacctaa ctgcgcccag tcgatgataa
    10801 ggccggtaga ggtgttatag tctgttagat tctgagttac ggtatttcgc gcgacatcta
    10861 atgctttgtt gttgaacaat agtttcgcac ccaggaagct tcatcggtgt gcaaagtgac
    10921 gaagaatggt gtgcaaagtg acgaagaatg gtgtgcaaag tgacgaagaa tggtgtgcaa
    10981 agtgacgaag aatgtagcag agttatccac aggctaaggg tgcagaaaat gaccaccttg
    11041 accccgagtg ggcgaagtga cgatgatgca gcatggtgtt tgatgtgacg aatctctttg
    11101 gggctactgg tgggtgaagt gacgaacgaa cgctcccctc tcctgcccga ccggcatccg
    11161 cagcgtgacc tgttcgtgtg cgacatcgtg gatgccgtcc ccaagggcga catgtcctca
    11221 atggagcatc ctgtcttttc actctccact aaaccggaca tgcgtccgcg gcggtacgag
    11281 cgcggcggca actggatcga gatttctccc tcgcgctacg gccttgcaac agtccacgac
    11341 cgcgatgtgc tgatctactg catcagccag tgcatgggcg cgttaaacga aggcaggaag
    11401 gtcgcccgca ccttgcgctt caaggcgcat gatcttctgg ttgcgactaa ccggcagacc
    11461 tcgggacgtg gctacgagct tctgaaggac gctctgcggc ggctgcaggg cacgcagatc
    11521 gagaccaacc ttcgccaagg cggcaaagag tatttcaggg tcttcgggct gatcgattct
    11581 gcggaagtgg tgcgcgagac acgcgacgga cgaatgcttg atgtcgagat cactctgtcg
    11641 gattgggtgt tcgatgctat cgagaacaac cacgtcctga cgctgaaccg gcgctacttc
    11701 ttcctacgca agccgctcga acgccgcctt tacgagctcg ctcggaagca ctgcgggcag
    11761 cagcaggagt ggcggatcgg actggatacg ctccgggaga agtgcggctc cggctcatcg
    11821 tccaaggaat tcaagcgcct ggtcggcaag atcatcgatg atgatgcgca gcacgactac
    11881 atgcctgact acgccctgac actcgatggt gaaattgtcg tggtccgtcc gaagaaggag
    11941 gcggcgcaga tacccctgcc cctcgcttcg tcagtaagcc tgcggatcaa cccggacagc
    12001 tacgacaagg caaggcagct cgcgcccgga tgggacgtac atgtgctaga aagcgaatgg
    12061 cgctcatggg tgatggacaa gagcatcgcc gtgaagaccc cggacgcgca tttcctaggc
    12121 ttctgcaaga agcgcggccc ctatgggcgc tgacgtttgc ttacacgcat ccatgcttgg
    12181 aacttatttg cacatgcatg catgcatcgc gccaagcatc aaaggatggt agcatgtaga
    12241 aaacacggag catgaggagc agtgaaccaa tgacctacat aatcggaatg gtgtcccaga
    12301 aaggcggagt cggtaagtcc accctcgccc gcatgatggc gcgggagttc gtctccggcg
    12361 gcctgagtgc gaagattgcg gacctcgata cacagcagca gacctgcacg aattgggccg
    12421 gtcggcgggc cgagaacgcc gtgacgcccg aacttcaggt gcagagcttc gctacggtga
    12481 aaacggcgct cgaagacgcc gcgcgcttcg acgccctgat cctggacggc aagccgaatg
    12541 cctcggaaca gacacttgag attgcgcagg ccgcagacct cattgtgatc ccgtccggcc
    12601 agacggtcga tgatctacat cctggcgtcg tcctggcgca cagcttgcgc aaaaagggga
    12661 tcgctcccat gaagatcgcc ttcgccatgt tcaagacgac cggcagcgac cgcgagaacc
    12721 atgcggcgcg acaatatctg atcgatgcag gttacgcggt actggacggc gacgtgccgg
    12781 tatcgacggc ttacggatcg gcctcggaca cagggaaggc gatcacagaa acctcgttcc
    12841 gcaccctgaa cagccgcgct gcaatgctcg cgcaaagcgt gatcgacaag ttggcagagc
    12901 ttcaggaaag gcaggtagcg tgatggcaac ggaccttgca aagctgaagc gtggaaaggg
    12961 gacgccgccg cctgcagacg atgtaccgga cgtgatcgcc gagaacgcgc gaagtgacga
    13021 ggcggaaccg ctgcgaccac tgcaggtgag gattccggcc agcatcttcg cggagtttag
    13081 cgagacggcc gggcgagagt tcggcttcac gcatggcgcg aagaagcagc tcttcctaaa
    13141 aatgtggaaa gcctacaagg cgctaagcat gcaatcatga ttgcatgctt agcgcaccag
    13201 cggagggttt agcgcaggag ctcgggaaga agaagccaag taccagttgt tgcgattgcg
    13261 gtaggaaagc cgccgaggca ggatcaggaa cctggacgct ccaaggcggg aacgccagat
    13321 gccacagatg ctccaccaag cctggcagcg ccgcacgagc tggtgcgggt gcctagaagt
    13381 gcaaaccact accaccgtta tggattgcgg tgcgtcctcc cggatgcaaa gccgctaatg
    13441 ataccgatgg aaggatcaat aaatctctca atatctggga taaggccttg cgttctaatc
    13501 atcgcaaaga tgcgaatttc ggcttccgga aaagtttctc gtagacgctc tgcgcaggca
    13561 taactagtgc ggcccatggt gagcacgtca tcaacaaccg tgatgcgttc aggctggaat
    13621 aaagtcgctg cgtcaacttc gatgctctcg aaatgttctg gccaaagagg ccgctcagca
    13681 gcaggggacg acgaggattt cctgacggcg tgcgtccgca ctaggaacgg cgcaacgtct
    13741 ctaccgaagc cactggccgc gagcacgtcg cagatgactc ggcttggcca tagtgctcca
    13801 tctgccaacg gggccgatct tgggacagga acaagcgtaa cgccatcgtt cagaaaaggc
    13861 cgcaagatgg cag
//
